Buffer effects on electric signals of light-excited bacteriorhodopsin mutants

European Biophysics Journal

Volumn 30, Issue 2, 2001, Pages 140-146

  Toth Boconadi R ^a   Der A ^a   Taneva, S G ^a   Tuparev, N P ^a   Keszthelyi, L ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

Author keywords

Activation enthalpy; Activation entropy; Orientation; Proton pump; Purple membrane

Indexed keywords

BACTERIORHODOPSIN; PROTON PUMP;

ARTICLE; CLUSTER ANALYSIS; ELECTRIC CONDUCTIVITY; ENTHALPY; ENTROPY; PURPLE MEMBRANE; SIGNAL TRANSDUCTION; TEMPERATURE DEPENDENCE;

AMINO ACID SUBSTITUTION; BACTERIORHODOPSINS; BUFFERS; CALORIMETRY; ELECTROCHEMISTRY; ENTROPY; GLYCYLGLYCINE; HALOBACTERIUM SALINARUM; KINETICS; LIGHT; MUTAGENESIS, SITE-DIRECTED; THERMODYNAMICS; TIME FACTORS;

ARRHENIUS; BACTERIA (MICROORGANISMS);

EID: 0034880847     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002490100132     Document Type: Article

References (21)

1. Rapid long-range proton diffusion along the surface of the purple membrane and delayed proton transfer into the bulk
2. Glutamic acid 204 is the terminal proton release group at the external surface of bacteriorhodopsin
3. Aspartic acids 96 and 85 play a central role in the function of bacteriorhodopsin as a proton pump
4. Mechanism of ptoton entry into the cytoplasmic section of the proton-conducting channel of bacteriorhodopsin
5. 15N shift anisotropy
6. 13C NMR study of the low pH forms of bacteriorhodopsin
7. Alternative translocation of protons and halide ions by bacteriorhodopsin
8. Existence of a proton transfer chain in bacteriorhodopsin: Participation of Glu-194 in the release of protons to the external surface
9. The role of arginines 82 and 227 in the bacteriorhodopsin photocycle
10. Charge motion in the D85N and D212N mutants of bacteriorhodopsin
11. Study of the photocycle and charge motions of the bacteriorhodopsin mutant D96N
12. Surface bound optical probes monitor proton translocation and surface potential changes during the bacteriorhodopsin photocycle
13. Proton migration along the membrane surface and retarded surface to bulk transfer
14. Photovoltage evidence that Glu-204 is the intermediate proton donor rather than the terminal proton release group in bacteriorhodopsin
15. Effect of pH buffer molecules on the light-induced currents from oriented purple membrane
16. Structural changes in bacteriorhodopsin during ion transport at 2 angstrom resolution
17. D38 is an essential part of the proton translocation pathway in bacteriorhodopsin
18. Effect of lipid surface charges on the purple-to-blue transition of bacteriorhodopsin
19. Inversion of proton translocation in bacteriorhodopsin mutants D85N, D85T, and D85/D95N
20. Buffer effects on electric signals of light excited bacteriorhodopsin
21. Protein structural changes at the CP surface is the cause of cooperativity in the bacteriorhodopsin photocycle