Neutral liposome-mediated delivery process of fluorescein-modified oligonucleotides in cultured human keratinocytes

Journal of Photochemistry and Photobiology B: Biology

Volumn 60, Issue 2-3, 2001, Pages 120-128

  Sasaki, Masako ^a   Hayashi, Junko ^a   Fujii, Masaki ^a   Koizumi, Kayo ^a   Fujita, Hitoshi ^a   Kobayashi, Masami ^b   Kawakubo, Yo ^c   Ito, Atsushi ^a  

^a TOKAI UNIVERSITY   (Japan)

^b UNIVERSITY OF TSUKUBA   (Japan)

^c TOKAI UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Antisense; Fluorescein; Keratinocyte; Liposome; Oligonucleotide; Photo induced binding

Indexed keywords

COMPLEMENTARY DNA; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; LIPOSOME; NUCLEASE; OLIGONUCLEOTIDE; PHOSPHATIDYLCHOLINE; PHOSPHOLIPID;

ARTICLE; CELL CULTURE; CELL NUCLEUS; CONTROLLED STUDY; COVALENT BOND; CYTOPLASM; DNA TRANSFER; ENCAPSULATION; FLUORESCENCE ANALYSIS; GENE EXPRESSION; GENE REPRESSION; HUMAN; HUMAN CELL; KERATINOCYTE; MEMBRANE; MODEL; MOLECULAR INTERACTION; OBSERVATION; PRIORITY JOURNAL; PROTECTION;

BINDING SITES; CELL NUCLEUS; CYTOPLASM; DEOXYRIBONUCLEASES; DNA; DNA, COMPLEMENTARY; DRUG DELIVERY SYSTEMS; FLUORESCEIN; HUMANS; KERATINOCYTES; LIPOSOMES; OLIGONUCLEOTIDES;

EID: 0034879635     PISSN: 10111344     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1011-1344(01)00135-X     Document Type: Article

References (30)

1. Potential of antisense technology in the treatment of immunological disorders
2. Gene inhibition using antisense oligodeoxynucleotides
3. Antisense oligonucleotides: A new therapeutic principle
4. A potential role for antisense oligonucleotide analogues in the development of oncogene targeted cancer chemotherapy
5. Rational design of sequence-specific oncogene inhibitors based on antisense and antigene oligonucleotides
6. Characteristics of oligonucleotide uptake in human keratinocyte cultures
7. Cationic lipid is not required for uptake and selective inhibitory activity of ICAM-1 phosphorothioate antisense oligonucleotides in keratinocytes
8. Increased oligonucleotide permeability in keratinocytes of artificial skin correlates with differentiation and altered membrane function
9. Flow cytometric and microscopic characterization of the uptake and distribution of phosphorothioate oligonucleotides in human keratinocytes
10. Characterization of membrane oligonucleotide-binding proteins and oligonucleotide uptake in keratinocytes
11. Oligonucleotide uptake in cultured keratinocytes: Influence of confluence, cationic liposomes, and keratinocyte cell type
12. Liposomes as a carrier for intracellular delivery of antisense oligonucleotides: A real or magic bullet?
13. A new liposomal formulation for antisense oligonucleotides with small size, high incorporation efficiency and good stability
14. Stability of antisense DNA oligonucleotide analogs in cellular extracts and sera
15. Rapid nuclear accumulation of injected oligodeoxyribonucleotides
16. Oligonucleotide analogues as potential chemotherapeutic agents
17. Antisense gene inhibition by oligonucleotides containing C-5 propyne pyrimidines
18. Biochemical and morphological characterization of growth and differentiation of normal human neonatal keratinocytes in a serum-free medium
19. Efficient methods for attaching non-radioactive labels to the 5′ ends of synthetic oligonucleotides
20. The anti-gene strategy: Control of gene expression by triplex-forming-oligonucleotides
21. Uptake of fluorescent oligonucleotides encapsulated in liposomes by cultured keratinocytes and their intracellular delivery process
22. Mechanism of oligonucleotide release from cationic liposomes
23. Intracellular oligonucleotide hybridization detected by fluorescence resonance energy transfer (FRET)
24. Phosphorothioate oligonucleotides bind in a non sequence-specific manner to the nucleolar protein C23/nucleolin
25. Effect of serum components on the physico-chemical properties of cationic lipid/oligonucleotide complexes and on their interactions with cells
26. Interaction of psoralen-derivatized oligonucleoside methylphosphonates with single-stranded DNA
27. Photochemical cross-linking of psoralen-derivatized oligonucleoside methylphosphonates to rabbit globin messenger RNA
28. Development of Antisense and Antigene Oligonucleotide Analogs
29. Photoactivatable antisense DNA: Suppression of ampicillin resistance in normally resistant Escherichia coli
30. Targeted mutagenesis in mammalian cells mediated by intracellular triple helix formation