Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin

Biophysical Journal

Volumn 81, Issue 3, 2001, Pages 1562-1569

  Webb, M R ^a   Corrie, J E T ^a  

^a NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE DIPHOSPHATE; COUMARIN; MYOSIN ADENOSINE TRIPHOSPHATASE; NUCLEOSIDE TRIPHOSPHATE; NUCLEOTIDE;

ARTICLE; CHEMICAL BINDING; CHEMICAL REACTION KINETICS; CONFORMATIONAL TRANSITION; FLUORESCENCE; IONIC STRENGTH; ISOMERISM; NONHUMAN; NUCLEOTIDE METABOLISM; PH MEASUREMENT; RABBIT; SIGNAL TRANSDUCTION; STEADY STATE; STRUCTURE ANALYSIS;

EID: 0034870958     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(01)75810-9     Document Type: Article

References (29)

1. Direct, real-time measurement of rapid inorganic phosphate release using a novel fluorescent probe and its application to actomyosin subfragment 1 ATPase
2. Mechanism of inorganic phosphate interaction with phosphate binding protein from Escherichia coli
3. Synthesis and fluorescent properties of a new photostable thiol reagent "BACM"
4. Thiol-reactive fluorescent probes for protein labelling
5. Synthesis and fluorescence properties of substituted 7-aminocoumarin-3-carboxylate derivatives
6. Interaction of actin and ADP with the head domain of smooth muscle myosin: Implications for strain-dependent ADP release in smooth muscle
7. Interaction of myosin subfragment 1 with fluorescent ribose-modified nucleotides: A comparison of vanadate trapping and SH1-SH2 cross-linking
8. The application of fluorescence and photosensitive analogues of guanine nucleotides to the function and structure of G-binding proteins
9. Fluorescence approaches to the study of the p21ras GTPase mechanism
10. The rate of mant-ADP release from cross-bridges of single skeletal muscle fibers
11. Laser dye stability. V. Effect of chemical substituents of bicyclic dyes upon photodegradation parameters
12. Dynamic interaction between actin and myosin subfragment 1 in the presence of ADP
13. Solution dynamics of p21ras proteins bound with fluorescent nucleotides: A time-resolved fluorescence study
14. Relationship between chemical and mechanical events during muscular contraction
15. New ribose-modified fluorescent analogs of adenine and guanine nucleotides available as substrates for various enzymes
16. The crystal structure of phosphate binding protein labeled with a coumarin fluorophore, a probe for inorganic phosphate
17. Proteolysis of smooth muscle myosin by Staphylococcus aureus protease: Preparation of heavy meromyosin and subfragment 1 with intact 20000-dalton light chains
18. Intrinsic fluorescence of actin
19. Formation of ribonucleotide 2′,3′-cyclic carbonates during conversion of ribonucleoside 5′-phosphates to diphosphates and triphosphates by the phosphoimidazolidate procedure
20. N-ras, the NRAS protooncogene product, is accompanied by a conformational change in the wild-type protein: Use of a single fluorescent probe at the catalytic site
21. Comparative single-molecule and ensemble myosin enzymology: Sulfoindocyanine ATP and ADP derivatives
22. Is there a rate limiting step before GTP-cleavage by H-ras p21
23. ADP dissociation from actomyosin subfragment 1 is sufficiently slow to limit the unloaded shortening velocity in vertebrate muscle
24. Active site comparisons highlight structural similarities between myosin and other P-loop proteins
25. Kinetic analysis of ATPase mechanisms
26. Separation of subfragment-1 isoenzymes from rabbit skeletal muscle myosin
27. Kinetics of interaction of 2′(3′)-O-(N-methylanthraniloyl)-ATP with myosin subfragment 1 and actomyosin subfragment 1: Characterization of two acto.S1.ADP complexes
28. The application of "excitation sculpting" in the construction of selective one-dimensional homonuclear coherence-transfer experiments