A high-throughput alphavirus-based expression cloning system for mammalian cells

Nature Biotechnology

Volumn 19, Issue 9, 2001, Pages 851-855

  Koller, Daniel ^a,b   Ruedl, Christiane ^a   Loetscher, Marcel ^a   Vlach, Jaromir ^a   Oehen, Stefan ^a   Oertle, Karin ^a   Schirinzi, Michel ^a   Deneuve, Estelle ^a   Moser, Rene ^c   Kopf, Manfred ^d   Bailey, James E ^b   Renner, Wolfgang ^a   Bachmann, Martin F ^a  

^a CYTOS BIOTECHNOLOGY AG   (Switzerland)

^b ETH ZURICH   (Switzerland)

^c IBR Inc   (Switzerland)

^d BASEL INSTITUTE FOR IMMUNOLOGY   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; DNA; FLUORESCENCE; GENES; MONOCLONAL ANTIBODIES; VIRUSES;

HARBORING;

BIOTECHNOLOGY;

CELL PROTEIN; COMPLEMENTARY DNA; HYBRID PROTEIN; LIGAND; MEMBRANE PROTEIN; MONOCLONAL ANTIBODY;

ALPHA VIRUS; ARTICLE; CELL CLONING; DNA LIBRARY; EXPRESSION VECTOR; FLUORESCENCE ACTIVATED CELL SORTER; GENE AMPLIFICATION; GENE EXPRESSION; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; REPLICON; SEQUENCE ANALYSIS; SINDBIS VIRUS; VIRUS ISOLATION; VIRUS PLAQUE; VIRUS RECOMBINANT;

ANIMALS; ANTIBODIES, MONOCLONAL; CELL LINE; CELL MEMBRANE; CELL SEPARATION; CELLS, CULTURED; CLONING, MOLECULAR; CRICETINAE; DNA, COMPLEMENTARY; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; LIGANDS; LUMINESCENT PROTEINS; MICE; MODELS, BIOLOGICAL; PROTEIN BINDING; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SINDBIS VIRUS; TIME FACTORS;

EID: 0034868955     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0901-851     Document Type: Article

References (28)

1. Drosophila genome takes flight
2. Molecular cloning of the CD2 antigen, the T-cell erythrocyte receptor, by a rapid immunoselection procedure
3. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system
4. Molecular cloning of two CD7 (T-cell leukemia antigen) cDNAs by a COS cell expression system
5. Efficient screening of retroviral cDNA expression libraries
6. Developments in expression cloning
7. Lentiviral vectors: Progress and potential
8. Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens
9. A simplified system for generating recombinant adenoviruses
10. Sindbis virus: An efficient, broad host range vector for gene expression in animal cells
11. The Alphaviruses: Gene expression, replication, and evolution
12. Alphaviruses as tools in neurobiology and gene therapy
13. Sindbis virus: An efficient, broad host range vector for gene expression in animal cells
14. Infection of human dendritic cells by a Sindbis virus replicon vector is determined by a single amino acid substitution in the E2 glycoprotein
15. Efficient gene delivery into adult cardiomyocytes by recombinant Sindbis virus
16. Translation of Sindbis virus mRNA: Effects of sequences downstream of the initiating codon
17. Infectious Sindbis virus transient expression vectors for studying antigen processing and presentation
18. Alphavirus-based expression vectors: Strategies and applications
19. Sindbis virus expression vectors: Packaging of RNA replicons by using defective helper RNAs
20. Homologous interference induced by Sindbis virus
21. High-efficiency identification of genes by functional analysis from a retroviral cDNA expression library
22. Expression cloning of the murine interferon gamma receptor cDNA
23. Applications of retrovirus-mediated expression cloning
24. Selection of RNA replicons capable of persistent noncytopathic replication in mammalian cells
25. Cloning by function: Expression cloning in mammalian cells
26. Interleukin-6 and its receptor: A paradigm for cytokines
27. Recombination between Sindbis virus RNAs
28. Myeloma based expression system for production of large mammalian proteins