Podosomes in osteoclast-like cells: Structural analysis and cooperative roles of paxillin, proline-rich tyrosine kinase 2 (Pyk2) and integrin αVβ3

Journal of Cell Science

Volumn 114, Issue 15, 2001, Pages 2775-2786

  Pfaff, M ^a   Jurdic, P ^a  

^a INSTITUT ALBERT BONNIOT   (France)

Author keywords

Cell adhesion; Cytoskeleton; Integrin; Osteoclast; Posodome

Indexed keywords

BETA3 INTEGRIN; F ACTIN; INTEGRIN; MONOCLONAL ANTIBODY; OSTEOCLAST DIFFERENTIATION FACTOR; PAXILLIN; POLYPEPTIDE; PROLINE; PROTEIN TYROSINE KINASE; TYROSINE; UNCLASSIFIED DRUG; VITRONECTIN RECEPTOR;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CELL ADHESION; CELL CONTACT; CELL DIFFERENTIATION; CELL INVASION; CELL LYSATE; CELL MIGRATION; CELL STRUCTURE; CHICKEN; CONTROLLED STUDY; CYTOPLASM; CYTOSKELETON; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE; IN VITRO STUDY; IN VIVO STUDY; MACROPHAGE; MONOCYTE; MULTINUCLEAR CELL; NONHUMAN; OSTEOCLAST; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION;

ACTINS; AMINO ACID SEQUENCE; ANIMALS; CARRIER PROTEINS; CELL ADHESION; CELLS, CULTURED; CHICKENS; CYTOSKELETAL PROTEINS; CYTOSKELETON; FLUORESCENT ANTIBODY TECHNIQUE; FOCAL ADHESION KINASE 2; MACROPHAGES; MEMBRANE GLYCOPROTEINS; MOLECULAR SEQUENCE DATA; MONOCYTES; OSTEOCLASTS; PAXILLIN; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN-TYROSINE KINASES; RANK LIGAND; RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B; RECEPTORS, VITRONECTIN; TYROSINE;

ANIMALIA; GALLUS GALLUS;

EID: 0034865951     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (61)

1. A homologue of the TNF receptor and its ligand enhance T-cell growth and dendritic cell function
2. Matrix metalloproteinases are obligatory for the migration of preosteoclasts to the developing marrow cavity of primitive long bones
3. Aggregation of mononucleated precursors triggers cell surface expression of αVβ3 integrin, essential to formation of osteoclast-like multinucleated cells
4. Fimbrin is a cytoskeletal protein that crosslinks F-actin in vitro
5. Ligand-dependent and -independent integrin focal contact localization: The role of the α chain cytoplasmic domain
6. Focal adhesions, contractility, and signaling
7. The talin head domain binds to integrin β subunit cytoplasmic tails and regulates integrin activation
8. F-actin aggregates in transformed cells contain α-actinin and fimbrin but apparently lack tropomyosin
9. Gelsolin deficiency blocks podosome assembly and produces increased bone mass and strength
10. Proteolytic activity of specialized surface protrusions formed at rosette contact sites of transformed cells
11. Expression of transformation-associated protease(s) that degrade fibronectin at cell contact sites
12. Ser752-Pro mutation in the cytoplasmic domain of integrin β3 subunit and defective activation of platelet integrin aIIbβ3 (glycoprotein IIbIIIa) in a variant of Glanzmann thrombasthenia
13. Altered distributions of the cytoskeletal proteins vinculin and α-actinin in cultured fibroblasts transformed by Rous sarcoma virus
14. The third helix of the Antennapedia homeodomain translocates through biological membranes
15. Pyk2 in osteoclasts is an adhesion kinase, localized in the sealing zone, activated by ligation of αVβ3 integrin, and phosphorylated by src kinase
16. β1 integrins and osteoclast function: Involvement in collagen recognition and bone resorption
17. Integrins: Versatility, modulation, and signaling in cell adhesion
18. The molecular architecture of focal adhesions
19. Altered localization and cytoplasmic domain-binding properties of tyrosine-phosphorylated β1 integrin
20. Regulation of fibronectin receptor distribution
21. Vitronectin receptor has a role in bone resorption, but does not mediate tight sealing zone attachment of osteoclasts to the bone surface
22. Stable association of PYK2 and p130cas in osteoclasts and their co-localization in the sealing zone
23. A calcium-dependent tyrosine kinase splice variant in human monocytes
24. The role of protein tyrosine phosphorylation in integrin-mediated gene induction in monocytes
25. Wiskott-Aldrich syndrome protein regulates podosomes in primary human macrophages
26. Cell substratum interaction of cultured avian osteoclasts is mediated by specific adhesion structures
27. Rous Sarcoma virus-transformed fibroblasts and cells of monocytic origin display a peculiar dot-like organization of cytoskeletal proteins involved in microfilament-membrane interaction
28. Vinculin, talin, and integrins are localized at specific adhesion sites of malignant B-lymphocytes
29. Mice lacking β3 integrins are osteosclerotic because of dysfunctional osteoclasts
30. Role of aVβ3 integrin in osteoclast migration and formation of the sealing zone
31. The podosomes of Rous Sarcoma virus transformed chondrocytes show peculiar ultrastructural organization
32. A functional link between dynamin and the actin cytoskeleton at podosomes
33. Regulation of integrin affinity states through an NPXY motif in the β subunit cytoplasmic domain
34. Identification of a talin-binding site in the integrin β3 subunit distinct from the NPLY regulatory motif of post-ligand binding functions: The talin N-terminal head domain interacts with the membrane-proximal cytoplasmic tail
35. Integrin β cytoplasmic domains differentially bind to cytoskeletal proteins
36. A combination of osteoclast differentiation factor and macrophage-colony stimulating factor is sufficient for both human and mouse osteoclast formation in vitro
37. Interplay between Rac and Rho in the control of substrate contact dynamics
38. Analysis of the binding of the src homology 2 domain of csk to tyrosine tyrosine-phosphorylated proteins in the suppression and mitotic activation of c-src
39. Identification of membrane-type matrix metalloproteinase MT1-MMP in osteoclasts
40. Distinct involvement of β3 integrin cytoplasmic domain tyrosine residues 747 and 759 in integrin-mediated cytoskeletal assembly and phosphotyrosine signaling
41. Differential signaling by the focal adhesion kinase and cell adhesion kinase β
42. Focal adhesion kinase and paxillin bind to peptides mimicking β integrin cytoplasmic domains
43. Direct association of protein-tyrosine phosphatase PTP-PEST with paxillin
44. FAK integrates growth-factor and integrin signals to promote cell migration
45. Multinucleated cells can continuously generate mononucleated cells in the absence of mitosis: A study of cells of the avian osteoclast lineage
46. Alpha-actinin-containing aggregates in transformed cells are highly dynamic structures
47. Paxillin association in vitro with integrin cytoplasmic domain peptides
48. Integrins isolated from Rous sarcoma virus-transformed chicken embryo fibroblasts
49. Rous sarcoma virus-transformed fibroblasts adhere primarily at discrete protrusions of the ventral membrane called podosomes
50. Paxillin: A cytoskeletal target for tyrosine kinases
51. Primary sequence of paxillin contains putative SH2 and SH3 domain binding motifs and multiple LIM domains: Identification of a vinculin and pp125FAK binding region
52. Paxillin LD4 motif binds PAK and PIX through a novel 95-kD ankyrin repeat, ARF-GAP protein: A role in cytoskeletal remodeling
53. The osteoclast clear zone is a specialized cell-extracellular matrix adhesion structure
54. The cell biology of osteoclast function
55. Unphosphorylated gelsolin is localized in regions of cell-substratum contact or attachment of Rous sarcoma virus-transformed rat cells
56. Detection of src homology 3-binding proteins, including paxillin, in normal and v-src-transformed Balb/c 3T3 cells
57. Osteoclast differentiation factor is a ligand for osteoprotegerin/ osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL
58. Gelsolin: Calcium- and polyphosphoinositide-regulated actin-modulating protein
59. Distinct functions of integrin α and β subunit cytoplasmic domains in cell spreading and formation of focal adhesions
60. Mutation of the cytoplasmic domain of the integrin β3 subunit
61. Immunocytochemical distribution of extracellular matrix receptors in human osteoclasts: A β3 integrin is colocalized with vinculin and talin in podosomes of osteoclastoma giant cells