Multi-primer target PCR for rapid identification of bovine DRB3 alleles

Animal Genetics

Volumn 32, Issue 4, 2001, Pages 219-221

  Ledwidge, Sarah A ^a,c   Mallard, B A ^a   Gibson, J P ^b   Jansen, G B ^a   Jiang, Z H ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b INTERNATIONAL LIVESTOCK RESEARCH INSTITUTE   (Kenya)

^c UNIVERSITY OF GUELPH   (Canada)

Author keywords

BoLA DRB3; Bovine lymphocyte antigen; Mastitis; MPT PCR; PCR RFLP

Indexed keywords

ALLELE; BREED; CANADA; COW; GENE IDENTIFICATION; MAMMALIAN GENETICS; MASTITIS; POLYMERASE CHAIN REACTION; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; SPECIFICITY;

ALLELES; ANIMALS; CATTLE; DNA; FEMALE; GENOTYPE; HISTOCOMPATIBILITY ANTIGENS CLASS II; POLYMERASE CHAIN REACTION;

ANIMALIA; BOVINAE; MAMMALIA;

EID: 0034862215     PISSN: 02689146     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2052.2001.00761.x     Document Type: Article

References (16)

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5. Alloreactive T-cell recognition of bovine major histocompatibility complex class II products defined by one-dimensional isoelectric focusing
6. The nucleotide sequence of bovine MHC class II DQB and DRB genes
7. Overlapping PCR for bidirectional PCR amplification of specific alleles: A rapid one-tube method for simultaneously differentiating homozygotes and heterozygotes
8. BoLA-DRB/DQB haplotypes as molecular markers of genetic susceptibility and resistance to bovine dermatophilosis
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10. Relationship between the bovine major histocompatibility complex (BoLA), erythrocyte markers and susceptibility to mastitis in Iceland cattle
11. Genetic impact on the risk of intramammary infection following Staphylococcus aureus challenge
12. Associations of the bovine major histocompatibility complex DRB3 (BoLA-DRB3) alleles with occurrence of disease and milk somatic cell score in Canadian dairy cattle
13. Identification and characterization of new BoLA-DRB3 alleles by heteroduplex analysis and direct sequencing
14. BoLA antigens are associated with increased frequency of persistent lymphocytosis in bovine leukemia virus infected cattle and with increased incidence of antibodies to bovine leukemia virus
15. Identification of BoLA-DRB3 in Japanese black cattle by PCR sequence-based typing
16. Extensive polymorphism of the BoLA-DRB3 gene distinguished by PCR-RFLP