PCR on cell lysates obtained from whole blood circumvents DNA isolation

Clinical Chemistry

Volumn 47, Issue 9, 2001, Pages 1701-1702

  De Vries, J E ^a   Wijnen, P A H M ^a   Hamulyak K ^a   Van Dieijen Visser, M P ^a   Bekers, O ^a  

^a MAASTRICHT UNIVERSITY MEDICAL CENTER   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

APOLIPOPROTEIN E; DNA;

ARTICLE; BLOOD; CELL LYSATE; GENE; GENOTYPE; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION;

EID: 0034859336     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/47.9.1701     Document Type: Article

References (13)

1. Direct PCR from whole blood, without DNA extraction
2. Direct PCR from CVS, blood lysates for detection of cystic fibrosis and Duchenne muscular dystrophy deletions
3. Direct PCR of washed blood cells
4. A simple method for DNA extraction from leukocytes for use in PCR
5. Optimized factor V gene mutation detection using buffy-coat direct PCR
6. Overview of principles and current uses of DNA probes in clinical and laboratory medicine
7. Interference of heparin with the polymerase chain reaction
8. PCR based diagnosis in the presence of 8% (v/v) blood
9. Apparent heparin interference with restriction enzyme digestion of genomic DNA
10. Inhibition of restriction endonucleases by common clinical anticoagulants
11. Genotyping and sequence analysis of apolipoprotein E isoforms
12. Restriction isotyping of human apolipoprotein E by gene amplification and cleavage with Hhal
13. Activated protein C resistance as an additional risk factor for thrombosis in protein C-deficient families