Site-directed mutagenesis of cysteine to threonine in Proteus vulgaris urease active site increases enzyme activity and stability

Biotechnology Letters

Volumn 23, Issue 15, 2001, Pages 1263-1267

  Jose, Joachim ^a   Lauter, Sven ^c   Stein, Martin A ^b  

^a SAARLAND UNIVERSITY   (Germany)

^b Axaron Bioscience AG   (Germany)

^c NONE

Author keywords

Active site; Proteus vulgaris; Site directed mutagenesis; Staphylococcus xylosus; Urease

Indexed keywords

BENZYLSULFONYL FLUORIDE; BIPYRIDINE DERIVATIVE; CYSTEINE; DISULFIDE; ENZYME VARIANT; THREONINE; UREASE;

AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME STABILITY; MICHAELIS CONSTANT; NONHUMAN; PH; PROTEUS VULGARIS; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

EID: 0034857197     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1010541513179     Document Type: Article

References (17)

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