Effects of cryopreservation of pronuclear-stage rabbit zygotes on the morphological survival, blastocyst formation, and full-term development after DNA microinjection

Molecular Reproduction and Development

Volumn 60, Issue 2, 2001, Pages 227-232

  Hochi, S ^a   Hirabayashi, M ^b   Hirao, M ^c   Kato, M ^a,b   Kobayashi, T ^a   Kimura, K ^a   Hirasawa, K ^c   Leibo, S P ^d   Ueda, M ^b  

^a SHINSHU UNIVERSITY   (Japan)

^b YS New Technology Institute Inc   (Japan)

^c Kitayama Labes Co Ltd   (Japan)

^d UNIVERSITY OF NEW ORLEANS   (United States)

Author keywords

Ethylene glycol; Freezing; Pronucleus; Transgenic rabbits; Vitrification

Indexed keywords

ACETAMIDE; DIMETHYL SULFOXIDE; DNA; ETHYLENE GLYCOL; PROPYLENE GLYCOL; SUCROSE;

ARTICLE; BLASTOCYST; CELL SURVIVAL; COLD SENSITIVITY; CONTROLLED STUDY; CRYOPRESERVATION; EMBRYO; FREEZING; FUSION GENE; MICROINJECTION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PRONUCLEUS; RABBIT; STRUCTURAL GENE; SURVIVAL RATE; TRANSGENIC ANIMAL; ZYGOTE;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; ANIMALS, NEWBORN; BLASTOCYST; CATTLE; CRYOPRESERVATION; DNA; EMBRYONIC AND FETAL DEVELOPMENT; FEMALE; GENES; HUMANS; MALE; MICROINJECTIONS; PROMOTER REGIONS (GENETICS); RABBITS; SURVIVAL RATE; ZYGOTE; ZYGOTE INTRAFALLOPIAN TRANSFER;

BOVINAE; ORYCTOLAGUS CUNICULUS;

EID: 0034855619     PISSN: 1040452X     EISSN: None     Source Type: Journal    
DOI: 10.1002/mrd.1082     Document Type: Article

References (49)

1. Expression of the murine wild-type tyrosinase gene in transgenic rabbits
2. Successful embryo transfer of cryopreserved and in-vitro fertilized rabbit oocytes
3. Survival of frozen rabbit embryos
4. Production of transgenic mice, rabbits and pigs by microinjection into pronuclei
5. Factors affecting the efficiency of introducing foreign DNA into mice by microinjecting eggs
6. Rabbit β-casein promoter directs secretion of human interleukin-2 into the milk of transgenic rabbits
7. Secretion of human erythropoietin by mammary gland explants from lactating transgenic rabbits
8. Effects of superovulation, culture and microinjection on development of rabbit embryos in vitro
9. Birth of piglets after transfer of embryos cryopreserved by cytoskeletal stabilization and vitrification
10. Inhibition of atherosclerosis development in cholesterol-fed human apolipoprotein A-I-transgenic rabbits
11. Overexpression of hepatic lipase in transgenic rabbits leads to a marked reduction of plasma high density lipoproteins and intermediate density lipoproteins
12. Factors affecting the survival of one- and two-cell rabbit embryos cryopreserved by vitrification
13. Production of transgenic rabbits, sheep and pigs by microinjection
14. Factors affecting the producing efficiency of transgenic rats
15. Effects of superovulatory protocols on the recovery and subsequent developmental potential of rabbit zygotes
16. Production of transgenic rabbits using centrifuged pronuclear zygotes
17. A comparative study on the integration of exogenous DNA into mouse, rat, rabbit, and pig genomes
18. Effects of cooling and warming rates during cryopreservation on survival of in vitro-produced bovine embryos
19. Effect of linoleic acid-albumin in the IVM and IVF media on survival of frozen-thawed pronuclear bovine zygotes
20. Effect of cooling and warming rates during vitrification on fertilization of in vitro-matured bovine oocytes
21. Open pulled straw (OPS) vitrification of porcine blastocysts: Simple procedure yielding excellent in vitro survival, but so far no piglets following transfer
22. Cryopreservation of in vitro-derived bovine blastocysts microinjected with foreign DNA at the pronuclear stage
23. High survival of rabbit morulae after vitrification in an ethylene glycol-based solution by a simple method
24. Piglets produced by transfer of vitrified porcine embryos after stepwise dilution of cryoprotectants
25. Effect of rapid addition and dilution of dimethyl sulfoxide and 37°C equilibration on viability of rabbit morulae thawed rapidly
26. Containerless vitrification of mammalian oocytes and embryos
27. Production of transgenic mice from cryopreserved fertilized ova
28. Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling
29. Kinetics of water loss and the likelihood of intracellular freezing in mouse ova
30. Cryopreservation of porcine embryos
31. Simple and efficient vitrification procedure for cryopreservation of mouse embryos
32. Function of milk protein gene 5′ flanking regions on human growth hormone gene
33. In vitro culture of rabbit ova from the single cell to the blastocyst stage
34. Factors affecting the survivability of bovine oocytes in droplets
35. Effects of membrane piercing and the type of pronuclear injection fluid on development of in vitro-produced bovine embryos
36. Cryobiology of rat embryos 1: Determination of zygote membrane permeability coefficients for water and cryoprotectants, their activation energies, and the development of improved cryopreservation methods
37. Chilling sensitivity of mammalian embryos
38. Preservation of hamster oocytes to assay the fertilizing capacity of human spermatozoa
39. Two-step freezing of two-cell rabbit embryos after partial dehydration at room temperature
40. In vitro and in vivo survival of rabbit embryos vitrified in EFS medium and increased PBS salt concentration
41. Stage-dependent viability of vitrified rabbit embryos
42. Developmental and tissue-specific expression of human CD4 in transgenic rabbits
43. Rabbit monoclonal antibodies: Generating a fusion partner to produce rabbit-rabbit hybridomas
44. Production of transgenic mice by microinjection of DNA into vitrified pronuclear-stage eggs
45. Production of transgenic rats using cryopreserved pronuclear-stage zygotes
46. Open pulled straw (OPS) vitrification: A new way to reduce cryoinjuries of bovine ova and embryos
47. Solvent effects on cytoskeltal organization and in vivo survival after freezing of rabbit oocytes
48. Osmotic and cryoprotective effects of mixture of DMSO and ethylene glycol on rabbit morulae
49. Stable production of human insulin-like growth factor 1 (IGF-1) in the milk of hemi- and homozygous transgenic rabbits over several generations