Role of clathrin in the regulated secretory pathway of pancreatic β-cells

Journal of Cell Science

Volumn 114, Issue 16, 2001, Pages 3059-3066

  Molinete, M ^a   Dupuis, S ^a   Brodsky, F M ^a   Halban, P A ^a  

^a UNIVERSITY OF GENEVA MEDICAL SCHOOL   (Switzerland)

Author keywords

C peptide; Clathrin; Insulin; Proinsulin; Regulated secretion; Trafficking

Indexed keywords

C PEPTIDE; CLATHRIN; HUB PROTEIN; INSULIN; PROINSULIN; PROTEINASE; UNCLASSIFIED DRUG;

ADENOVIRUS; ANIMAL CELL; ARTICLE; CELL MATURATION; CELL POPULATION; CELL SECRETION; CELL SELECTION; CONTROLLED STUDY; EXOCYTOSIS; FLUORESCENCE ACTIVATED CELL SORTER; GRANULE CELL; IMMUNOFLUORESCENCE; INSULIN RELEASE; MALE; NONHUMAN; PANCREAS ISLET BETA CELL; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; QUANTITATIVE ASSAY; RAT; REGULATORY MECHANISM; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SECRETORY GRANULE; VIRUS RECOMBINANT;

ADENOVIRIDAE; ANIMALS; C-PEPTIDE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CLATHRIN; CLATHRIN HEAVY CHAINS; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; GREEN FLUORESCENT PROTEINS; INSULIN; ISLETS OF LANGERHANS; LUMINESCENT PROTEINS; PEPTIDE FRAGMENTS; PROINSULIN; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN TRANSPORT; RATS; SECRETORY VESICLES;

ADENOVIRIDAE; ANIMALIA;

EID: 0034846068     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (45)

1. Predominance of clathrin light chain LCb correlates with the presence of a regulated secretory pathway
2. ADP-ribosylation factor 6 and endocytosis at the apical surface of Madin-Darby canine kidney cells
3. Protein sorting and secretion granule formation in regulated secretory cells
4. Sorting and storage during secretory granule biogenesis: Looking backward and looking forward
5. Protein discharge from immature secretory granules displays both regulated and constitutive characteristics
6. Protein transport from the secretory to the endocytic pathway in mammalian cells
7. Importance of cell-matrix interactions in rat islet beta-cell secretion in vitro: Role of alpha6beta1 integrin
8. Living with clathrin: Its role in intracellular membrane traffic
9. The AP-1 adaptor complex binds to immature secretory granules from PC12 cells, and is regulated by ADP-ribosylation factor
10. Interaction of furin in immature secretory granules from neuroendocrine cells with the AP-1 adaptor complex is modulated by casein kinase II phosphorylation
11. Sorting and processing of secretory proteins
12. Resistance of the insulin crystal to lysosomal proteases: Implications for pancreatic B-cell crinophagy
13. A simplified system for generating recombinant adenoviruses
14. Clathrin and adaptors
15. Proinsulin processing in the rat insulinoma cell line INS after overexpression of the endoproteases PC2 or PC3 by recombinant adenovirus
16. Adaptors for clathrin-mediated traffic
17. Clathrin
18. Mannose 6-phosphate receptors are sorted from immature secretory granules via adaptor protein AP-1, clathrin, and syntaxin 6-positive vesicles
19. Protein targeting via the 'constitutive-like' secretory pathway in isolated pancreatic islets: Passive sorting in the immature granule compartment
20. Distinct molecular mechanisms for protein sorting within immature secretory granules of pancreatic beta-cells
21. Differential sorting of lysosomal enzymes out of the regulated secretory pathway in pancreatic beta-cells
22. Regulation of clathrin assembly and trimerization defined using recombinant triskelion hubs
23. A dominant-negative clathrin mutant differentially affects trafficking of molecules with distinct sorting motifs in the class II major histocompatibility complex (MHC) pathway
24. Sorting human beta-cells consequent to targeted expression of green fluorescent protein
25. Novel, non-crinophagic, degradation of connecting peptide in transformed pancreatic beta cells
26. Macro and micro-domains in the endocrine pancreas
27. A clathrin-coated, Golgi-related compartment of the insulin secreting cell accumulates proinsulin in the presence of monensin
28. Nonconverted, amino acid analog-modified proinsulin stays in a Golgi-derived clathrin-coated membrane compartment
29. Insulin, not C-peptide (proinsulin), is present in crinophagic bodies of the pancreatic B-cell
30. Clathrin-immunoreactive sites in the Golgi apparatus are concentrated at the trans pole in polypeptide hormone-secreting cells
31. Proteolytic maturation of insulin is a post-Golgi event which occurs in acidifying clathrin-coated secretory vesicles
32. pH-independent and -dependent cleavage of proinsulin in the same secretory vesicle
33. A test of clathrin function in protein secretion and cell growth
34. Clathrin: A role in the intracellular retention of a Golgi membrane protein
35. Genetic and biochemical characterization of clathrin-deficient Saccharomyces cerevisiae
36. Newly synthesized proinsulin/insulin and stored insulin are released from pancreatic B cells predominantly via a regulated, rather than a constitutive, pathway
37. Stimulation by ATP of proinsulin to insulin conversion in isolated rat pancreatic islet secretory granules
38. Differences in aggregation properties and levels of the neural cell adhesion molecule (NCAM) between islet cell types
39. Clathrin-coated vesicle formation and protein sorting: An integrated process
40. A role for clathrin in the sorting of vacuolar proteins in the Golgi complex of yeast
41. Yeast clathrin has a distinctive light chain that is important for cell growth
42. Clathrin-coated vesicular transport of secretory proteins during, the formation of ACTH-containing secretory granules in AtT20 cells
43. Protease-activated receptor-1 down-regulation. A mutant Hela cell line suggests novel requirements for par1 phosphorylation and recruitment to clathrin-coated pits
44. Protein traffic from the secretory pathway to the endosomal system in pancreatic beta-cells
45. Des-(27-31)C-peptide. A novel secretory product of the rat pancreatic beta cell produced by truncation of proinsulin connecting peptide in secretory granules