Defined sequence segments of the small heat shock proteins HSP25 and αB-crystallin inhibit actin polymerization

European Journal of Biochemistry

Volumn 268, Issue 7, 2001, Pages 2083-2090

  Wieske, Martin ^a,b   Benndorf, Rainer ^b,c   Behlke, Joachim ^b   Dölling, Rudolf ^d   Grelle, Gerlinde ^b   Bielka, Heinz ^b   Lutsch, Gudrun ^b  

^a FRITZ HABER INSTITUT DER MAX PLANCK GESELLSCHAFT   (Germany)

^b MAX DELBRÜCK CENTER FOR MOLECULAR MEDICINE   (Germany)

^c UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^d Biosyntan GmbH   (Germany)

Author keywords

Actin polymerization; Electron microscopy; Fluorescence spectroscopy; Phosphorylation; Small heat shock proteins

Indexed keywords

ACTIN; ALPHAB CRYSTALLIN; HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 25; OLIGOPEPTIDE; UNCLASSIFIED DRUG;

ACTIN POLYMERIZATION; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; ELECTRON MICROSCOPY; FLUORESCENCE SPECTROSCOPY; MOLECULAR INTERACTION; MOUSE; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION;

ACTINS; AMINO ACID SEQUENCE; ANIMALS; CONSENSUS SEQUENCE; CONSERVED SEQUENCE; CRYSTALLINS; HEAT-SHOCK PROTEINS; MICE; MICROSCOPY, ELECTRON; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; NEOPLASM PROTEINS; PEPTIDE FRAGMENTS; PHOSPHORYLATION; POLYMERS; SEQUENCE ALIGNMENT; SERINE; STRUCTURE-ACTIVITY RELATIONSHIP;

EID: 0034843626     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02082.x     Document Type: Article

References (57)

1. Four small Drosophila heat shock proteins are related to each other and to mammalian α-crystallin
2. αB-crystallin is a small heat shock protein
3. Expression and function of the low-molecular-weight heat shock proteins
4. Structure and function of small heat shock proteins
5. The quaternary structure of bovine α-crystallin
6. Dynamic changes in the structure and intracellular locale of the mammalian low-molecular-weight heat shock protein
7. Supra-molecular structure of the recombinant small heat shock protein hsp25
8. Phosphorylation and supramolecular organization of murine small heat shock protein HSP25 abolish its actin polymerization-inhibiting activity
9. 2 chain of bovine α-crystallin
10. The in vivo phosphorylation sites of bovine αB-crystallin
11. Identification of the phosphorylation sites of the murine small heat shock protein hsp25
12. Human HSP27 is phosphorylated at serines 78 and 82 by heat shock and mitogen-activated kinases that recognize the same amino acid motif as S6 kinase II
13. Phosphorylation of αB-crystallin in response to various types of stress
14. α-Crystallin can function as a molecular chaperone
15. Small heat shock proteins are molecular chaperones
16. Cardiac alpha-crystallin. III. Involvement during heart ischemia
17. αB-crystallin in cardiac tissue. Association with actin and desmin filaments
18. Chaperone activity of α-crystallins modulates intermediate filament assembly
19. Sense and antisense modification of glial αB-crystallin production results in alterations of stress fiber formation and thermoresistence
20. α-Crystallin stabilizes actin filaments and prevents cytochalasin-induced depolymerization in a phosphorylation-dependent manner
21. Abundance and location of the small heat shock proteins HSP25 and αB-crystallin in rat and human heart
22. A 25-kD inhibitor of actin polymerization is a low molecular mass heat shock protein
23. The Saccharomyces cerevisiae small heat shock protein Hsp26 inhibits actin polymerization
24. Induction of Chinese hamster HSP27 gene expression in mouse cells confers resistance to heat shock. HSP27 stabilization of the microfilament organization
25. Modulation of actin microfilament dynamics and fluid phase pinocytosis by phosphorylation of heat shock protein 27
26. Variation in expression of hsp27 messenger ribonucleic acid during the cycle of the seminiferous epithelium and co-localization of hsp27 and microfilaments in Sertoli cells of the rat
27. Basolateral membrane-associated 27-kDa heat shock protein and microfilament polymerization
28. Heat shock protein 27 kDa expression and phosphorylation regulates endothelial cell migration
29. Protein phosphatase inhibitors and heat preconditioning prevent Hsp27 dephosphorylation. F-actin disruption and deterioration of morphology in ATP-depleted endothelial cells
30. In vivo evaluation of hsp27 as an inhibitor of actin polymerization: Hsp27 limits actin stress fiber and focal adhesion formation after heat shock
31. Molecular cloning, sequencing and expression in Escherichia coli of the 25-kDa growth-related protein of Ehrlich ascites tumor and its homology to mammalian stress proteins
32. Laser desorption ionisation of proteins with molecular masses exceeding 10.000 daltons
33. Purification of muscle actin
34. Measurement of rate constants for actin filament elongation in solution
35. Fluorimetry study of N-(1-pyrenyl) iodoacetamide-labelled F-actin
36. Inhibition of actin polymerization by a synthetic dodecapeptide patterned on the sequence around the actin-binding site of cofilin
37. G- to F-actin modulation by single amino acid substitution in the actin binding site of actobindin and thymosin beta 4
38. Evidence that a 27-residue sequence is the actin-binding site of ABP-120
39. Identification and characterization of an actin-binding site of CapZ
40. Structure-function relationships in the ezrin family and the effect of tumor-associated point mutations in neurofibromatosis 2 protein
41. The interfaces of actin and Acanthamoeba actobindin. Identification of a new actin-binding motif
42. The small heat shock-related protein-20 is an actin-associated protein
43. Crystal structure of a small heat-shock protein
44. Small heat-shock protein family: Function in health and disease
45. Ischemic acute renal failure induces differential expression of small heat shock proteins
46. HSP27 phosphorylation-mediated resistance against actin fragmentation and cell death induced by oxidative stress
47. HSP27 expression regulates CCK-induced changes of the actin cytoskeleton in CHO-CCK-A cells
48. Early and delayed tolerance to simulated ischemia in heat-preconditioned endothelial cells: A role for HSP27
49. Association of α-crystallin with actin in cultured lens cells
50. Phosphorylation of αB-crystallin in mitotic cells and identification of enzymatic activities responsible for phosphorylation
51. Identification of MAPKAP kinase 2 as a major enzyme responsible for the phosphorylation of the small heat shock proteins
52. Phosphorylation of αB-crystallin and HSP27 is induced by similar stressors in HeLa cells
53. Synapsin I bundles F-actin in a phosphorylation-dependent manner
54. Mitosis-specific phosphorylation causes 83K non-muscle caldesmon to dissociate from microfilaments
55. Reactivation of phosphorylated actin depolymerizing factor and identification of the regulatory site
56. Phosphorylation of Ser-3 of cofilin regulates its essential function on actin