Overexpression and purification of Aspergillus aculeatus β-mannosidase and analysis of the integrated gene in Aspergillus oryzae

Journal of Bioscience and Bioengineering

Volumn 92, Issue 2, 2001, Pages 131-137

  Kanamasa, Shin ^a   Takada, Goro ^a   Kawaguchi, Takashi ^a   Sumitani, Jun Ichi ^a   Arai, Motoo ^a  

^a Osaka Prefecture University   (Japan)

Author keywords

Aspergillus aculeatus; Aspergillus oryzae; Overexpression; Quantitative PCR; Region III; mannosidase

Indexed keywords

CHROMATOGRAPHY; ELECTROPHORESIS; FLUORESCENCE; GENES; PROTEINS; ULTRAVIOLET RADIATION;

OVEREXPRESSION;

PURIFICATION;

BETA MANNOSIDASE; GENE PRODUCT; PROTEIN MANB; UNCLASSIFIED DRUG;

ARTICLE; ASPERGILLUS; ASPERGILLUS ORYZAE; BACTERIUM COLONY; COLUMN CHROMATOGRAPHY; ENZYME PURIFICATION; EXPRESSION VECTOR; FLUORESCENCE; GENE OVEREXPRESSION; GENETIC ANALYSIS; GENETIC CODE; MOLECULAR WEIGHT; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROMOTER REGION; PROTEIN BINDING; SOUTHERN BLOTTING;

ASPERGILLUS ACULEATUS; ASPERGILLUS ORYZAE; BACTERIA (MICROORGANISMS);

EID: 0034842564     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1263/jbb.92.131     Document Type: Article

References (19)

1. Human β-mannosidase cDNA characterization and first identification of a mutation associated with human β-mannosidosis
2. Caprine β-mannosidase: Sequencing and characterization of the cDNA and identification of the molecular defect of caprine β-mannosidase
3. Molecular cloning and characterization of bovine β-mannosidase
4. Comparison of a β-glucosidase and a β-mannosidase from the gyperthermophilic archaeon Pyrococcus furiosus. Purification, characterization, gene cloning, and sequence analysis
5. Isolation and identification of a cellulolytic enzyme producing microorganism
6. Purification and properties of a β-mannosidase from Aspergillus aculeatus
7. Cloning and sequencing of the cDNA encoding β-glucosidase 1 from Aspergillus aculeatus
8. Cloning and sequencing of β-mannosidase gene from Aspergillus aculeatus
9. Cloning, nucleotide sequence, and transcriptional analysis of Aspergillus aculeatus no. F-50 cellobiohydrolase I (cbhl) gene
10. Improvement of promoter activity by the introduction of multiple copies of the conserved region III sequence, involved in the efficient expression of Aspergillus oryzae amylase-encoding genes
11. Construction of a promoter probe vector autonomously maintained in Aspergillus and characterization of promoter regions derived from A. niger and A. oryzae genomes
12. Integrative transformation of Aspergillus oryzae with a plasmid containing the Aspergillus nidulans argB gene
13. Studies on transformation of Escherichia coli with plasmids
14. Differential spectrophotometry of purine compounds by means of specific enzymes
15. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
16. Cloning and sequencing of an endoglucanase gene from Scopulariopsis brevicaulis TOF-1212, and its expression in Saccharomyces cerevisiae
17. Cloning, characterization and overproduction of nuclease S1 gene (nucS) from Aspergillus oryzae
18. Molecular and enzymic properties of recombinant 1,2-α-mannosidase from Aspergillus saitoi overexpressed in Aspergillus oryzae cells