Effect of exogenous lysine on the expression of early cephamycin C biosynthetic genes and antibiotic production in Nocardia lactamdurans MA4213

Applied Microbiology and Biotechnology

Volumn 56, Issue 5-6, 2001, Pages 670-675

  Abramov, Yoram ^a   Nadjari, Michel ^a   Weinstein, Daniel ^a   Ben Shachar, Inbar ^a   Plotkin, Vladimir ^a   Ezra, Yossef ^a  

^a HADASSAH HEBREW UNIVERSITY MEDICAL CENTER   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

2 AMINOADIPIC ACID; AMINO ACID; BETA LACTAM; CARBON; CEPHAMYCIN C; CYSTEINE; LYSINE; LYSINE 6 AMINOTRANSFERASE; MESSENGER RNA; NITROGEN;

ANTIBIOTICS; BIOTECHNOLOGY;

ACTINOBACTERIA; ANTIBIOTIC BIOSYNTHESIS; ARTICLE; BACTERIUM CULTURE; BIOSYNTHESIS; CONTROLLED STUDY; DRUG MANUFACTURE; ENZYME ACTIVITY; FERMENTATION; GENE EXPRESSION; NOCARDIA; NOCARDIA LACTAMDURAN; NONHUMAN; POLYMERIZATION; SUPPLEMENTATION;

BACTERIAL PROTEINS; CEPHAMYCINS; CULTURE MEDIA; GENE EXPRESSION REGULATION, BACTERIAL; L-LYSINE 6-TRANSAMINASE; LYSINE; NOCARDIA; TRANSAMINASES;

ACTINOBACTERIA (CLASS); AMYCOLATOPSIS LACTAMDURANS; BACTERIA (MICROORGANISMS); NOCARDIA; UNCULTURED ACTINOMYCETE;

EID: 0034840439     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530100715     Document Type: Article

References (23)

1. Overexpression of the lat gene in Nocardia lactamdurans from strong heterologous promoters results in a very high levels of lysine-6-aminotransferase and up to two-fold increase in cephamycin C production
2. A gene encoding lysine 6-aminotransferase, which forms the beta-lactam precursor alpha-aminoadipic acid, is located in the cluster of cephamycin biosynthetic genes in Nocardia lactamdurans
3. The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from Acremonium chrysogenum and Penicillium chrysogenum
4. Analysis of the promoter region of saf, a Streptomyces griseus gene that increases production of extracellular enzymes
5. Delta-1-piperideine-6-carboxylate dehydrogenase, a new enzyme that forms alpha-aminoadipate in Streptomyces clavuligerus and other cephamycin C-producing actinomycetes
6. Effect of primary metabolites on secondary metabolism
7. The nine genes of the Nocardia lactamdurans cephamycin cluster are transcribed into large mRNAs from three promoters, two of them located in a bidirectional promoter region
8. Unexpected enhancement of beta-lactam antibiotic formation in Streptomyces clavuligerus by very high concentrations of exogenous lysine
9. Sporulation and the production of serine protease and cephamycin C by Streptomyces lactamdurans
10. L-lysine ε-aminotransferase involved in cephamycin C synthesis in Streptomyces lactamdurans
11. Metabolic engineering of cephalosporin biosynthesis in Streptomyces clavuligerus
12. Allophane increases the protein levels of several cephamycin biosynthetic enzymes in Nocardia lactamdurans
13. What is secondary metabolism?
14. Isolation of RNA using guanidinium salts
15. Lysine catabolism in Streptomyces spp. is primarily through cadaverine: Beta-lactam producers also make alpha-aminoadipate
16. Cloning and location of a gene governing lysine epsilon-aminotransferase, an enzyme initiating beta-lactam biosynthesis in Streptomyces spp.
17. Precursor flux control through targeted chromosomal insertion of the lysine epsilon-aminotransferase (lat) gene in cephamycin C biosynthesis
18. Regulation of cephamycin C synthesis, aspartokinase, dihydrodipicolinic acid synthetase, and homoserine dehydrogenase by aspartic acid family amino acids in Streptomyces clavuligerus
19. The pcd gene encoding piperideine-6-carboxyl-ate dehydrogenase involved in biosynthesis of alpha-aminoadipic acid is located in the cephamycin cluster of Streptomyces clavuligerus
20. Induction of L-lysine epsilon-aminotransferase by L-lysine in Streptomyces clavuligerus, producer of cephalosporins
21. Partial purification, characterization and nitrogen regulation of the lysine epsilon-aminotransferase of Streptomyces clavuligerus
22. Effects of replacement of promoters and modification of the leader peptide region of the amy gene of Streptomyces griseus on synthesis and secretion of α-amylase by Streptomyces lividans