Enhancer-binding proteins HrpR and HrpS interact to regulate hrp-encoded type III protein secretion in Pseudomonas syringae strains

Journal of Bacteriology

Volumn 183, Issue 19, 2001, Pages 5589-5598

  Hutcheson, S W ^a   Bretz, J ^a   Sussan, T ^a   Jin, S ^a   Pak, K ^a  

^a UNIVERSITY OF MARYLAND   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEIN; ENHANCER BINDING PROTEIN; GENE PRODUCT; PROTEIN HRPR; PROTEIN HRPS; PROTEIN KINASE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE ACTIVATION; GENE CONTROL; GENE TRANSLOCATION; GENETIC TRANSCRIPTION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PATHOGENESIS; PATHOGENICITY; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DOMAIN; PROTEIN INTERACTION; PROTEIN SECRETION; PSEUDOMONAS SYRINGAE; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; TWO HYBRID SYSTEM;

BACTERIAL PROTEINS; BASE SEQUENCE; DNA-BINDING PROTEINS; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; MOLECULAR SEQUENCE DATA; OPERON; PROMOTER REGIONS (GENETICS); PSEUDOMONAS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA POLYMERASE SIGMA 54; SIGMA FACTOR; TRANSCRIPTION FACTORS; TWO-HYBRID SYSTEM TECHNIQUES;

ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS SYRINGAE;

EID: 0034836583     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.19.5589-5598.2001     Document Type: Article

References (66)

1. + exchange in tobacco
2. Using the two-hybrid system to detect protein-protein interactions
3. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu
4. The Pseudomonas syringae avrRpt2 gene product promotes pathogen virulence from inside plant cells
5. Coupling flagellar gene expression to flagellar assembly in Salmonella enterica serovar typhimurium and Escherichia coli
6. Association of the Arabidopsis CTR1 Raf-like kinase with ETR1 and ERS ethylene receptors
7. Pseudomonas syringae Hrp type III secretion system and effector proteins
8. Characterization of the hrpC and hrpRS operons of Pseudomonas syringae pathovars syringae, tomato, and glycinea and analysis of the ability of hrpF, hrpG, hrcC, hrpT, and hrpV mutants to elicit the hypersensitive response and disease in plants
9. 54-dependent transcription
10. Evidence for the secretion of Chlamydia trachomatis CopN by a type III secretion mechanism
11. High efficiency transformation in yeast
12. Expression of the Pseudomonas syringae avirulence protein AvrB in plant cells alleviates its dependence on the hypersensitive response and pathogenicity (Hrp) secretion system in eliciting genotype-specific hypersensitive cell death
13. Regulation of capsule synthesis: Modification of the two-component paradigm by an accessory unstable regulator
14. The hrpRS locus of Pseudomonas syringae pv. phaseolicola constitutes a complex regulatory unit
15. The predicted protein product of a pathogenicity locus from Pseudomonas syringae pv. phaseolicola is homologous to a highly conserved domain of several prokaryotic regulatory proteins
16. The alternative sigma factor RpoN is required for hrp activity in Pseudomonas syringae pv. maculicola and acts at the level of hrpL transcription
17. Virulence of the phytopathogen Pseudomonas syringae pv. maculicola is rpoN dependent
18. Bacteria in the leaf ecosystem with emphasis on Pseudomonas syringae: A pathogen, ice nucleus, and epiphyte
19. Type III secretion genes identify a putative virulence locus of Chlamydia
20. Molecular cloning of a Pseudomonas syringae pv. syringae gene cluster that enables Pseudomonas fluorescens to elicit the hypersensitive response in tobacco
21. Type III protein secretion systems in bacterial pathogens of animals and plants
22. Currents concepts of active defense in plants
23. The hrp cluster of Pseudomonas syringae: A pathogenicity island encoding a type III protein translocation complex?
24. Molecular analysis of avirulence gene avrRpt2 and identification of a putative regulatory sequence common to all known Pseudomonas syringae avirulence genes
25. Identification of a pathogenicity island, which contains genes for virulence and avirulence, on a large native plasmid in the bean pathogen Pseudomonas syringae pv. phaseolicola
26. Autogenous control of PspF, a constitutively active enhancer-binding protein of Escherichia coli
27. Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon
28. Improved broad host range plasmids for DNA cloning in gram negative bacteria
29. The hrcC and hrpN operons of Erwinia chrysanthemi EC16 are flanked by plcA and homologs of hemolysin/adhesion genes and accompanying activator/transporter genes
30. The major dimerization determinants of the nitrogen regulatory protein NtrC from enteric bacteria lies at its carboxy-terminal domain
31. Bacterial avirulence genes
32. 54-dependent transcriptional activator
33. Analysis of the Streptomyces coelicolor sigE gene reveals the existence of a subfamily of eubacterial RNA polymerase σ factors involved in the regulation of extracytoplasmic functions
34. Flagella and motility
35. The sigma 54 bacterial enhancer-binding protein family: Mechanism of action and phylogenetic relationship of their functional domains
36. Characterization of pPT23B, the plasmid involved in syringolide production by Pseudomonas syringae pv. tomato PT23
37. Eukaryotic fatty acid acylation drives plasma membrane targeting and function of several type III effector proteins from Pseudomonas syringae
38. A proposed architecture for the central domain of the bacterial enhancer-binding proteins based on secondary structure prediction and fold recognition
39. Communication modules in bacterial signaling proteins
40. The N-terminal domain of the prokaryotic enhancer-binding protein XylR is a specific intramolecular repressor
41. Phenotypic expression of Pseudomonas syringae avr genes in E. coli is linked to the activities of the hrp-encoded secretion system
42. Negative regulation of hrp genes in Pseudomonas syringae by HrpV
43. Plant and environmental sensory signals control the expression of hrp genes in Pseudomonas syringae pv. phaseolicola
44. The avrRpm1 gene of Pseudomonas syringae pv. maculicola is required for virulence on Arabidopsis
45. Conserved domains in bacterial regulatory proteins that respond to environmental stimuli
46. Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells
47. Molecular characterization and hrp-dependence of the avirulence gene avrPto from Pseudomonas syringae pv. tomato
48. Characterization of the promoter of avirulence gene D from Pseudomonas syringae pv. tomato
49. 54-dependent regulators: Derepression as a control mechanism
50. Molecular characterization of cloned avirulence genes from race 0 and 1 of Pseudomonas syringae pv. glycinea
51. Broad host range and promoter selection vectors for bacteria that interact with plants
52. The Avr (effector) proteins HrmA (HopPsyA) and AvrPto are secreted in culture from Pseudomonas syringae pathovars via the Hrp (type III) protein secretion system in a temperature- and pH-sensitive manner
53. The gene coding for the Hrp pilus structural protein is required for type III secretion of Hrp and Avr proteins in Pseudomonas syringae pv. tomato
54. Regulation of hrp genes and type III protein secretion in Erwinia amylovora by HrpX/HrpY, a novel two component system, and HrpS
55. hrpL activates Erwinia amylovora hrp gene transcription and is a member of the ECF subfamily of sigma factors
56. Identification of Pseudomonas syringae pathogens of Arabidopsis and a bacterial locus determining avirulence on both Aribidopsis and soybean
57. BEAUTY-X: Enhanced BLAST searches for DNA queries
58. Unusual oligomerization required for activity of NtrC, a bacterial enhancer-binding protein
59. Identification of a putative alternate sigma factor and characterization of a multicomponent regulatory cascade controlling the expression of Pseudomonas syringae pv. syringae Pss61 hrp and hrmA genes
60. A single promoter sequence recognized by a newly identified alternate sigma factor directs expression of pathogenicity and host range determinants in Pseudomonas syringae
61. Organization and environmental regulation of the Pseudomonas syringae pv. syringae 61 hrp cluster
62. Modulation of host immune responses, induction of apoptosis and inhibition of NF-κB activation by Bordetella type III secretion system