Effects of pressure on the activity and spectroscopic properties of carboxyl proteinases: Apparent correlation of pepstatin-insensitivity and pressure response

European Journal of Biochemistry

Volumn 268, Issue 3, 2001, Pages 645-655

  Fujiwara, Satoshi ^a   Kunugi, Shigeru ^a,b   Oyama, Hiroshi ^b   Oda, Kohei ^b  

^a Kyoto Institute of Technology   (Japan)

^b KYOTO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Activity; Aspartic proteinase; Carboxyl proteinase; Fluorescence; Pressure dependence

Indexed keywords

CARBOXYL GROUP; MYOGLOBIN; PEPSIN A; PEPSTATIN; PEPTIDE; PROTEINASE; PROTEINASE A; PSEUDOMONAPEPSIN; UNCLASSIFIED DRUG; XANTHOMONAPEPSIN;

AQUEOUS SOLUTION; ARTICLE; AUTOLYSIS; CIRCULAR DICHROISM; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME SPECIFICITY; ENZYME SUBSTRATE; NONHUMAN; PRESSURE; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DENATURATION; PSEUDOMONAS; SACCHAROMYCES CEREVISIAE; SWINE; ULTRAVIOLET SPECTROPHOTOMETRY; XANTHOMONAS;

ANIMALS; ASPARTIC ENDOPEPTIDASES; CIRCULAR DICHROISM; HYDROGEN-ION CONCENTRATION; HYDROLYSIS; KINETICS; PEPSIN A; PEPSTATINS; PRESSURE; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS; SACCHAROMYCES CEREVISIAE; SPECTROMETRY, FLUORESCENCE; SPECTROPHOTOMETRY; SWINE; THERMODYNAMICS; TRYPTOPHAN; TYROSINE; ULTRAVIOLET RAYS; XANTHOMONAS;

EID: 0034836475     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.01917.x     Document Type: Article

References (55)

1. Proteins under pressure. The influence of high hydrostatic pressure on structure, function and assembly of proteins and protein complexes
2. Exploiting the effects of high hydrostatic pressure in biotechnological application
3. High pressure effects on protein structure and function
4. Action of hydrostatic pressure on proteins: Emergence of high pressure biotechnology, potential pharmaceutical and medical applications
5. Modification of biopolymer functions by high pressure
6. Effect of pressure on EcoRI reaction
7. Effect of pressure on subtilisin catalysis: Hydrolysis and peptide synthesis
8. Effects of high-pressure on the activity and spectroscopic properties of carboxypeptidase Y
9. The effect of high pressure on thermolysin
10. Single-point amino acid substitutions at the 119th residue of thermolysin and their pressure-induced activation
11. Pressure-assisted cold-denaturation of carboxypeptidase Y
12. Studies of the formation and stability of the complex between Streptomyces proteinaceous metalloprotease inhibitor (SMPI) and thermolysin
13. Activity and stability of a neutral protease from Vibrio sp. (vimelysin) in a pressure-temperature gradient
14. The effect of pressure on protease-catalyzed peptide formation
15. Introduction of high pressure to food processing: Preferential proteolysis of β-lactoglobulin in milk whey
16. Protease-catalyzed peptide formation under high pressure
17. Proteolytic enzymes
18. Pepstatin-insensitive carboxyl proteinases. Structure and function of the aspartic proteinase
19. Pepstatin-insensitive carboxyl proteinases
20. The specificity and mechanism of pepsin action
21. The mechanism of the catalytic action of pepsin and related acid proteinases
22. Purification of yeast proteinases. Part III. Isolation and physicochemical properties of yeast proteinase A and C
23. Purification and properties of proteinase A from yeast
24. Purification and properties of a pepstatin-insensitive carboxyl proteinase from a gram-negative bacterium
25. Substrate specificity, and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp, no. 101
26. The primary structure of pepstatin-insensitive carboxyl proteinase produced by Pseudomonas sp., no. 110
27. Cloning, nucleotide sequence, and expression of an isovaleryl pepstatin-insensitive carboxyl proteinase gene from Pseudomonas so. 101
28. Identification of carboxyl residues in pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. 101 that participate in catalysis and substrate binding
29. Purification and properties of an S-PI (pepstatin Ac)-insensitive carboxyl proteinase from a Xanthomonas sp.
30. Cloning and expression of an isovaleryl pepstatin-insensitive carboxyl peptidase gene from Xanthomonas sp. T-22
31. Identification of catalytic residues of pepstatin-insensitive carboxyl proteinase from prokaryotes by site-directed mutagenesis
32. Substrate specificities and kinetic properties of proteinase A from the yeast Saccharomyces cerevisiae and the development of a novel substrate
33. Fluorimetric assays of proteolytic enzymes
34. Fourth derivative UV-spectroscopy of proteins under high pressure, I. Factors affecting the fourth derivative spectrum of the aromatic amino acids
35. Fourth derivative UV-spectroscopy of proteins under high pressure. II. Applications to reversible structural changes
36. Pressure dissociation and conformational drift of the beta dimer of tryptophan synthase
37. Pressure-induced unfolding/refolding of ribonuclease A: Static and kinetic Fourier transform infrared spectroscopy study
38. Contribution of the carbohydrate moiety to conformational stability of carboxypeptidase Y. High pressure study
39. Structure of ethanol-inhibited porcine pepsin at 2-Å resolution and binding of the methyl ester of phenylalanyl-diiodotyrosine to the enzyme
40. Molecular and crystal structures of monoclinic porcine pepsin refined at 1.8 Å resolution
41. The three dimensional structure at 2.4 Å resolution of glycosylated proteinase A from the lysosome-like vacuole of Saccharomyces cerevisiae
42. Evolution in the structure and function of aspartic proteases
43. Domain flexibility in aspartic proteinases
44. Revised 2.3 Å structure of procine pepsin: Evidence for a flexible subdomain
45. Comparison of the specificity of the aspartic proteinases towards internally consistent sets of oligopeptide substrates
46. Subsite preferences of pepstatin-insensitive carboxyl proteinases from bacteria
47. Subsite preferences of pepstatin-insensitive carboxyl proteinases from prokaryotes: Kumamolysin, a thermostable pepstatin-insensitive carboxyl proteinase
48. Conformational instability of the N- and C-terminal lobes of porcine pepsin in natural and alkaline solutions
49. Prediction of protein secondary structure using 3D-1D compatibility algorithm