The study of oxidative stress in freshly isolated Ca2+-tolerant cardiomyocytes from the adult rat

Toxicology in Vitro

Volumn 15, Issue 4-5, 2001, Pages 283-287

  Remiao F ^a   Carmo, H ^a   Carvalho, F ^a   Bastos, M L ^a  

^a UNIVERSITY OF PORTO   (Portugal)

Author keywords

Glutathione; Isolated cardiomyocytes; Isoproterenol; Oxidative stress; Tert Butylhydroperoxide

Indexed keywords

CALCIUM ION; CATECHOLAMINE; COPPER; GLUTATHIONE; GLUTATHIONE PEROXIDASE; GLUTATHIONE REDUCTASE; GLUTATHIONE TRANSFERASE; ISOPRENALINE; TERT BUTYL HYDROPEROXIDE;

ARTICLE; CARCINOMA CELL; CARDIOTOXICITY; CELL COUNT; CELL ISOLATION; CELL SHAPE; CELL VIABILITY; COLON ADENOCARCINOMA; COMPARATIVE STUDY; ENZYME ACTIVITY; HEART MUSCLE CELL; INCUBATION TIME; NONHUMAN; OXIDATIVE STRESS;

ANIMALS; CALCIUM; CELL COUNT; CELL SURVIVAL; DOSE-RESPONSE RELATIONSHIP, DRUG; GLUTATHIONE; GLUTATHIONE PEROXIDASE; GLUTATHIONE REDUCTASE; GLUTATHIONE TRANSFERASE; ISOPROTERENOL; MALE; MYOCARDIUM; OXIDATIVE STRESS; RATS; RATS, WISTAR; TERT-BUTYLHYDROPEROXIDE;

EID: 0034830263     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0887-2333(01)00022-4     Document Type: Article

References (27)

1. Hydroperoxide-induced nucleotide degradation and adenosine formation in isolated rat cardiomyocytes
2. Morphology and metabolism of intact muscle cells isolated from adult rat heart
3. Biochemical and toxicological properties of the oxidation products of catecholamines
4. A new method for preparation of isolated single adult myocytes
5. Nonspecific proteases: A new approach to the isolation of adult cardiocytes
6. Effect of d-amphetamine repeated administration on rat antioxidant defences
7. An improved procedure for the hight-yeld preparation of intact beating heart from the adult rat biochemical and morphologic study
8. Isolation of calcium tolerant myocytes from adult rat hearts: Review of the literature and description of a method
9. In vitro studies of single isolated beating heart cells
10. 2+-resistent myocytes from the adult rat
11. Use of isolated adult myocytes to evaluate cardiotoxicity. I. Sugar uptake and protein synthesis
12. Calcium tolerant ventricular myocytes prepared by preincubation in a "KB medium"
13. The effects of insulin and anoxia on the metabolism of isolated mature rat cardiac myocytes
14. Roles of collagenases and other proteolytic enzymes in the dispersal of animal tissues
15. A simple method for calibrating collagenase/pronase E ratio to optimize heart cell isolation
16. The dissociation and aggregation of cells from organ rudiments of the early chick embriyo
17. Isoprenaline stimulation of cyclic AMP production by isolated cells from adult rat myocardium
18. A rapid technique for the isolation and purification of adult cardiac muscle cells having resoiratory control and a tolerance to calcium
19. Application of in vitro model systems to the study od cardiovascular toxicity
20. Inhibition of glutathione reductase by isoproterenol oxidation products
21. Simultaneous determination of reduced and oxidized glutathione in freshly isolated rat hepatocytes and cardiomyocytes by HPLC with electrochemical detection
22. Cardiotoxicity studies using freshly isolated calcium-tolerant cardiomyocytes from adult rat
23. Cellular glutathione and the response of adult rat heart myocytes to oxidant stress
24. Preparation of beating heart cells from adult rats
25. Improved isolation of cardiomyocytes by trypsination in addition to collagenase treatment
26. A primary culture system of adult rat heart cells for the study of toxicologic agents