The cysteine synthase complex from plants: Mitochondrial serine acetyltransferase from Arabidopsis thaliana carries a bifunctional domain for catalysis and protein-protein interaction

European Journal of Biochemistry

Volumn 268, Issue 3, 2001, Pages 686-693

  Wirtz, Markus ^a   Berkowitz, Oliver ^a   Droux, Michel ^b   Hell, Rüdiger ^a  

^a LEIBNIZ INSTITUTE OF PLANT GENETICS AND CROP PLANT RESEARCH IPK   (Germany)

^b SANOFI   (France)

Author keywords

Amino acid; Catalysis; Modeling; Protein protein interaction; Structure

Indexed keywords

ACYLTRANSFERASE; CYSTEINE; CYSTEINE SYNTHASE; ENZYME; FUNCTIONAL GROUP; HEXAPEPTIDE; SERINE ACETYLTRANSFERASE;

ARABIDOPSIS; ARTICLE; BETA SHEET; BIOSYNTHESIS; CATALYSIS; CLONING; COMPUTER ANALYSIS; CRYSTAL STRUCTURE; ENZYME ASSAY; GENETIC COMPLEMENTATION; MODEL; NONHUMAN; NUCLEOTIDE REPEAT; NUCLEOTIDE SEQUENCE; OLIGOMERIZATION; PLANT; PRIORITY JOURNAL; PRISM; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; REGULATORY MECHANISM; SITE DIRECTED MUTAGENESIS; TWO HYBRID SYSTEM;

ACETYLTRANSFERASES; AMINO ACID SEQUENCE; ARABIDOPSIS; CARBON-OXYGEN LYASES; CATALYSIS; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; CYSTEINE SYNTHASE; DATABASES, FACTUAL; DNA MUTATIONAL ANALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENETIC COMPLEMENTATION TEST; KINETICS; MITOCHONDRIA; MODELS, GENETIC; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; MUTAGENESIS, SITE-DIRECTED; PLANTS; PLASMIDS; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; SACCHAROMYCES CEREVISIAE PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SERINE O-ACETYLTRANSFERASE; SOFTWARE; TWO-HYBRID SYSTEM TECHNIQUES;

EID: 0034825333     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.01920.x     Document Type: Article

References (34)

1. Biosynthesis of cysteine
2. Molecular physiology of plant sulfur metabolism
3. Sulfate transport and assimilation in plants
4. Isoform-dependent differences in feedback regulation and sub-cellular localization of serine-acetyltransferase involved in cysteine biosynthesis from Arabidopsis thaliana
5. Genomic and functional characterization of the oas gene family encoding O-acetylserine (thiol) lyases, enzymes catalyzing the final step in cysteine biosynthesis in Arabidopsis thaliana
6. Purification and characterization of cysteine synthetase, a bifunctional protein complex, from Salmonella typhimurium
7. Measurement of serine acetyltransferase activity in crude plant extracts by a coupled assay system using cysteine synthase
8. Purification and characterization of O-acetylserine (thiol) lyase from spinach chloroplasts
9. Purification and kinetic properties of serine acetyltransferase free of O-acetylserine (thiol) lyase from spinach chloroplasts
10. Molecular cloning and characterization of a plant serine acetyltransferase playing a regulatory role in cysteine biosynthesis from watermelon
11. Cysteine synthesis in plants: Protein-protein interactions of serine acetyltransferase from Arabidopsis thaliana
12. Interactions between serine acetyltransferase and O-acetylserine (thiol) lyase in higher plants. Structural and kinetic properties of the free and bound enzymes
13. Eight bacterial proteins, including UDP-N-acetylglucosamine acyltransferase (LpxA) and three other trans-ferases of Escherichia coli, consist of a six-residue periodicity theme
14. The novel hexapeptide motif found in the acyltransferase lpxA and lpxD of lipid A biosynthesis is conserved in various bacteria
15. The PROSITE dictionary of sites and patterns in proteins, its current status
16. O-acetyltransferases for chloramphenicol and other natural products
17. A left-handed parallel b-helix in the structure of UDP-N-acetylglucosamine acyltransferase
18. The active site of Escherichia coli UDP±N-acetylglucosamine acyltransferase
19. The serine acetyltransferase from Escherichia coli. Over-expression, purification and preliminary crystallographic analysis
20. Kinetic mechanism of serine transacetylase from Salmonella typhimurium
21. Molecular Cloning. A Laboratory Manual
22. Cysteine biosynthesis in plants: Isolation and functional characterization of a cDNA encoding serine acetyltransferase from Arabidopsis thaliana
23. Cloning and characterisation of an Arabidopsis thaliana cDNA clone encoding an organellar isoform of serine acetyltransferase
24. New method for generating deletions and gene replacements in Escherichia coli
25. Molecular cloning and expression analysis of mitochondrial and plastidic isoforms of cysteine synthase (O-acetylserine(thiol)lyase) from Arabidopsis thaliana
26. Pleiotropy in a cysteine-requiring mutant of Salmonella typhimurium resulting from altered protein-protein interaction
27. Cysteine biosynthesis: Serine transacetylase and O-acetylserine sulfhydrylase
28. Protein interaction cloning in yeast: Identification of mammalian proteins that react with the leucine zipper of Jun
29. Three-dimensional structure of tetrahydrodipicolinate N-succinyltransferase
30. Structure of the hexapeptide xenobiotic acetyltransferase from Pseudomonas aeruginosa
31. Three-dimensional structure of O-acetylserine sulfhydrylase from Salmonella typhimurium
32. Serine acetyltransferase from Escherichia coli is a dimer of trimers
33. A left-handed β-helix revealed by the crystal structure of a carbonic anhydrase from the archeon Methanosarcina thermophila