Identification of functional regions of guanylate cyclase-activating protein 1 (GCAP1) using GCAP1/GCIP chimeras

Biological Chemistry

Volumn 382, Issue 8, 2001, Pages 1179-1188

  Li, N ^a   Sokal, I ^a   Bronson, J D ^a   Palczewski, K ^a   Baehr, W ^a  

^a UNIVERSITY OF UTAH SCHOOL OF MEDICINE   (United States)

Author keywords

Ca2+ binding proteins; Guanylate cyclase; Guanylate cyclase activating proteins; Guanylate cyclase inhibitory proteins; Retinal photoreceptors

Indexed keywords

CALCIUM BINDING PROTEIN; CALCIUM ION; CALMODULIN BINDING PROTEIN; GUANYLATE CYCLASE; GUANYLATE CYCLASE ACTIVATOR; GUANYLATE CYCLASE INHIBITOR; HELIX LOOP HELIX PROTEIN; MUTANT PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CHIMERA; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME INHIBITION; FROG; GENE CONSTRUCT; NONHUMAN; PHOTORECEPTOR CELL; PHYSIOLOGY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN MOTIF; PROTEIN PURIFICATION; RETINA; SEQUENCE HOMOLOGY; VERTEBRATE;

AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CALCIUM; CALCIUM-BINDING PROTEINS; CATTLE; CELLS, CULTURED; EYE PROTEINS; GUANYLATE CYCLASE; GUANYLATE CYCLASE-ACTIVATING PROTEINS; INSECTS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; RECEPTORS, CELL SURFACE; RECOMBINANT PROTEINS; RODS (RETINA); TRANSCRIPTION FACTORS; XENOPUS PROTEINS;

EID: 0034820024     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.2001.148     Document Type: Article

References (43)

1. Three-dimensional structure of guanylyl cyclase activating protein-2, a calcium-sensitive modulator of photoreceptor guanylyl cyclases
2. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
3. Constitutive activation of photoreceptor guanylate cyclase by Y99C mutant of GCAP-1
4. Inactivation of EF hands makes GCAP-2 (p24) a constitutive activator pf photoreceptor guanylyl cyclase by preventing a Ca-induced 'activator-to-inhibitor' transition
5. Regulation of photoreceptor membrane guanylyl cyclases by guanylyl cyclase activator proteins
6. Recoverin: A calcium sensitive activator of retinal rod guanylate cyclase
7. 2+-binding protein activating photoreceptor guanylyl cyclase
8. Functional consequences of a rod outer segment membrane quanylate cyclase (ROS-GC1) gene mutation linked with Leber's congenital amaurosis
9. Calcium modulation of bovine photoreceptor guanylate cyclase
10. Three-dimensional structure of recoverin, a calcium sensor in vision
11. Purification and physiological evaluation of a guanylate cyclase activating protein from retinal rods
12. Guanylyl cyclase activating protein: A calcium-sensitive regulator of phototransduction
13. Molecular characterization of a third member of the guanylyl cyclase-activating protein subfamily
14. Gene array and expression of mouse retina guanylate cyclase activating proteins 1 and 2
15. Recoverin's role: Conclusion withdrawn
16. Regulation of sensitivity in vertebrate rod photoreceptors by calcium
17. Identification of proximate regions in a complex of retinal guanylyl cyclase 1 and guanylyl cyclase activating protein-1 by a novel mass spectrometry based method
18. Mapping sites in guanylyl cyclase activating protein-1 required for regulation of photoreceptor membrane guanylyl cyclases
19. 2+-binding protein related to mammalian guanylate cyclase activating proteins
20. The role of calcium-binding sites in S-modulin function
21. Guanylate cyclase-activating proteins (GCAPs) set the flash sensitivity of rod photoreceptors
22. Mapping functional domains of the guanylate cyclase regulator protein, GCAP-2
23. Functional reconstitution of photoreceptor guanylate cyclase with native and mutant forms of guanylate cyclase activating protein 1
24. Localization of guanylate cyclase activating protein 2 in mammalian retinas
25. Molecular cloning and characterization of retinal photoreceptor guanylyl cyclase activating protein (GCAP)
26. Calcium binding proteins in the retina: Structure, function, and the etiology of human visual diseases
27. Preparation of retinal rod outer segments
28. A mutation in guanylate cyclase activator 1A (GUCA1A) in an autosomal dominant cone dystrophy pedigree mapping to a new locus on chromosome 6p21.1
29. Retinal-specific guanylate cyclase gene mutations in Leber's congenital amaurosis
30. 2+-binding proteins in the retina
31. Photoreceptor guanylate cyclases: A review
32. Changes in biological activity and folding of guanylate cyclase-activating protein 1 as a function of calcium
33. CHELATOR: An improved method for computing metal ion concentrations in physiological solutions
34. Identification of a domain in guanylyl cyclase-activating protein 1 that interacts with a complex of guanylyl cyclase and tubulin in photoreceptors
35. A null mutation in the photoreceptor guanylate cyclase gene causes the retinal degeneration chicken phenotype
36. Molecular characterization of the chicken GCAP gene array and analysis of GCAP1, GCAP2, and GC1 gene expression in normal and rd chicken pineal gland
37. GCAP1(Y99C) mutant is constitutively active in autosomal dominant cone dystrophy
38. Identification of a guanylyl cyclase-activating protein-binding site within the catalytic domain of retinal guanylyl cyclase 1
39. Conformational changes in guanylyl cyclase-activating protein 1 (GCAP1) and its tryptophan mutants as a function of calcium concentration
40. The human GCAP1 and GCAP2 genes are arranged in a tail-to-tail array on the short arm of chromosome 6 (p21.1)
41. A rapid and efficient method for site-directed mutagenesis using one-step overlap extension PCR
42. Disruption of a retinal guanylyl cyclase gene leads to cone-specific dystrophy and paradoxical rod behavior