Incomplete refolding of a fragment of the N-terminal domain of pig muscle 3-phosphoglycerate kinase that lacks a subdomain: Comparison with refolding of the complementary C-terminal fragment

European Journal of Biochemistry

Volumn 268, Issue 6, 2001, Pages 1851-1860

  Szilágyi, Andrea N ^a   Kotova, Nina V ^b   Semisotnov, Gennady V ^b   Vas, Mária ^a,c  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b INSTITUTE OF PROTEIN RESEARCH   (Russian Federation)

^c INSTITUTE OF ENZYMOLOGY   (Hungary)

Author keywords

Folding intermediates; Phosphoglycerate kinase; Protein domains

Indexed keywords

3 PHOSPHOGLYCERIC ACID; 8 ANILINO 1 NAPHTHALENESULFONIC ACID; MUSCLE ENZYME; PHOSPHOGLYCERATE KINASE; PHOSPHORUS DERIVATIVE; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ANIMAL TISSUE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; CONTROLLED STUDY; DIMERIZATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; FLUORESCENCE; GEL PERMEATION CHROMATOGRAPHY; HYDROPHOBICITY; INCUBATION TIME; MICROCALORIMETRY; NONHUMAN; PHASE TRANSITION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; STRUCTURE ANALYSIS; SWINE;

ANIMALS; CALORIMETRY, DIFFERENTIAL SCANNING; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROLYSIS; MUSCLES; PHOSPHOGLYCERATE KINASE; PROTEIN FOLDING; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; SWINE;

EID: 0034819229     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02060.x     Document Type: Article

References (48)

1. Evolutionary conservation of the substrate binding cleft of 3-phosphoglycerate kinases
2. Sequence, structure and activity of phosphoglycerate kinase: A possible hinge-bending enzyme
3. Sequence and structure of yeast phosphoglycerate kinase
4. Crystal structure of the binary complex of pig muscle phosphoglycerate kinase and its substrate 3-phospho-D-glycerate
5. Structure of the ADP complex of the 3-phosphoglycerate kinase from Bacillus stearothermophilus at 1.65 Å
6. Synergistic effects of substrate-induced conformational changes in phospho-glycerate kinase activation
7. Closed structure of phospho-glycerate kinase from Thermotoga maritima reveals the catalytic mechanism and determinants of thermal stability
8. Nuclear magnetic resonance studies of isolated structural domains of yeast phosphoglycerate kinase
9. Efficient expression and characterization of isolated structural domains of yeast phosphoglycerate kinase generated by site-directed mutagenesis
10. Role of the C-terminal helix in the folding and stability of yeast phospho-glycerate kinase
11. An engineered amino-terminal domain of yeast phosphoglycerate kinase with native-like structure
12. Is the structure of the N domain of phosphoglycerate kinase affected by isolation from the intact molecule?
13. Structure of a kinetic protein folding intermediate by equlibrium amide exchange
14. Thermodynamic study of phospho-glycerate kinase from Thermotoga maritima and its isolated domains: Reversible thermal unfolding monitored by differential scanning calorimetry and circular dichroism spectroscopy
15. Limited proteolysis of 3-phospho-glycerate kinase without loss of enzymic activity
16. Reactivation of 3-phosphoglycerate kinase from its proteolytic fragments
17. Sequential domain refolding of pig muscle 3-phosphoglycerate kinase: Kinetic analysis of reactivation
18. Refolding of pig muscle and yeast 3-phosphoglycerate kinases and of their proteolytic fragments
19. Folding and functional complementation of engineered fragments from yeast phosphoglycerate kinase
20. The phosphate group of 3-phosphoglycerate accounts for conformational changes occurring on binding to 3-phosphoglycerate kinase: Enzyme inhibition and thiol reactivity studies
21. On the role of heme in the formation of the structure of cytochrome c
22. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
23. Adenine nucleotides affect the binding of 3-phosphoglycerate to pig muscle 3-phosphoglycerate kinase
24. Estimation of protein secondary structure from circular dichroism
25. Use of a trypsin-pulse method to study the refolding pathway of ribonuclease
26. The slow-refolding step of phosphoglycerate kinase as monitored by pulse proteolysis
27. Probing the partly folded states of proteins by limited proteolysis
28. The conformation of horse heart apocytochrome c
29. Sequential mechanism of refolding of carbonic anhydrase B
30. Tertiary interactions in the folding pathway of hen lysozyme: Kinetic studies using fluorescent probes
31. Kinetics of interaction of partially folded proteins with a hydrophobic dye: Evidence that molten globule character is maximal in early folding intermediates
32. Study of the `molten globule' intermediate state in protein folding by a hydrophobic fluorescent probe
33. Refolding of recombinant sulphonated procathepsin S and of reduced chicken cystatin; implications for renaturation experiments
34. Structural mapping of an aggregation nucleation site in a molten globule intermediate
35. Association-induced folding of globular proteins
36. alpha→beta transition of beta-lactoglobulin as evidenced by heteronuclear NMR
37. Role of hydrophobic interactions in yeast phosphoglycerate kinase stability
38. Proteins can adopt totally different folded conformations
39. Conversion of yeast phospho-glycerate kinase into amyloid-like structure
40. Scanning microcalorimetry in studying temperature-induced changes in proteins
41. Structural, kinetic, and calorimetric characterization of the cold-active phosphoglycerate kinase from the antarctic Pseudomonas sp. TACII18
42. Analytical gel chromatography of proteins
43. Estimation of molecular size and molecular weights of biological compounds by gel filtration
44. Occurrence of transient multimeric species during the refolding of a monomeric protein
45. Evidence for a molten globule state as a general intermediate in protein folding
46. Structures of folding intermediates
47. How molten is the molten globule?
48. 2.0 Å resolution structure of a ternary complex of pig muscle phosphoglycerate kinase containing 3-phospho-D-glycerate and the nucleotide Mn adenylylimidodiphosphate