Transition state complexes of the Klebsiella pneumoniae nitrogenase proteins: Spectroscopic properties of aluminium fluoride-stabilized and beryllium fluoride-stabilized MgADP complexes reveal conformational differences of the Fe protein

European Journal of Biochemistry

Volumn 268, Issue 3, 2001, Pages 809-818

  Miller, Richard W ^a   Eady, Robert R ^b   Fairhurst, Shirley A ^b   Gormal, Carol A ^b   Smith, Barry E ^b  

^a Ecovale Research   (United States)

^b JOHN INNES CENTRE   (United Kingdom)

Author keywords

Conformation; Fluorescent analogues; Metal fluoride complexes; Nitrogenase; Nucleotides

Indexed keywords

2'(3') O (N METHYLANTHRANILOYL)ADENOSINE DIPHOSPHATE; ADENOSINE; ADENOSINE DERIVATIVE; ADENOSINE DIPHOSPHATE; ADENOSINE DIPHOSPHATE MAGNESIUM; ADENOSINE PHOSPHATE; ADENOSINE PHOSPHATE MAGNESIUM; ADENOSINE TRIPHOSPHATE; BACTERIAL PROTEIN; FLUORIDE ION; METAL COMPLEX; MYOSIN; NITROGENASE; PROTEIN KP2; SOLVENT; TETRAFLUOROBERYLLIUM ION; TRIFLUOROALUMINUM ION; TYROSINE; UNCLASSIFIED DRUG;

ARTICLE; BINDING SITE; CATALYSIS; COMPLEX FORMATION; CONFORMATIONAL TRANSITION; ENERGY TRANSFER; HYDROLYSIS; KLEBSIELLA PNEUMONIAE; PHOSPHORUS NUCLEAR MAGNETIC RESONANCE; POLARIZATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN ISOLATION;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; ALUMINUM COMPOUNDS; BERYLLIUM; BINDING SITES; CATALYSIS; ELECTRON TRANSPORT; FLUORIDES; IRON; KLEBSIELLA PNEUMONIAE; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, CHEMICAL; NITROGENASE; PROTEIN BINDING; PROTEIN CONFORMATION; SPECTROPHOTOMETRY; TIME FACTORS; ULTRAVIOLET RAYS;

EID: 0034817799     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.01941.x     Document Type: Article

References (38)

1. Mechanism of molybdenum nitrogenase
2. Structural basis of biological nitrogen fixation
3. Nitrogenase: Substrate binding and activation
4. Nitrogenase structure and function: A biochemical-genetic perspective
5. Crystallographic structure and functional implications of the nitrogenase molybdenum-iron protein from Azotobacter vinelandii
6. X-Ray crystal structure of the nitrogenase molybdenum-iron protein from Clostridium pasteurianum at 3.0 Å resolution
7. New insights into structure-function relationships in nitrogenase: A 1.6 Å resolution X-ray crystallographic study of Klebsiella pneumoniae MoFe-protein
8. Nitrogenase of Klebsiella pneumoniae. Kinetics of the dissociation of oxidized iron protein from molybdenum-iron protein: Identification of the rate-limiting step for substrate reduction
9. Nitrogenase of Klebsiella pneumoniae. Kinetic on the Fe protein involving reduction by sodium dithionite, the binding of MgADP and a conformation change that alters the reactivity of the 4Fe-4S centre
10. Electron transfer from the iron protein to the [8Fe-(7/8)S] clusters of the molybdenum-iron protein
11. Changes in the midpoint potentials of the nitrogenase metal centers as a result of iron protein-molybdenum-iron protein complex formation
12. 13P NMR study of ADP and ATP interactions with the Fe protein of Klebsiella pneumoniae
13. Crystallographic structure of the nitrogenase iron protein from Azotobacter vinelandii
14. Conformational variability in structures of the nitrogenase iron proteins from Azotobacter vinelandii and Clostridium pasteurianum
15. Nitrogenase: A nucleotide-dependent molecular switch
16. -
17. Formation and characterization of a transition state complex of Azotobacter vinelandii nitrogenase
18. Aluminum fluoride inhibition of nitrogenase. Stabilization a nucleotide-Fe-protein-MoFe-protein complex
19. Nitrogenase of Klebsiella pneumoniae: Kinetics of formation of the transition-state complex and evidence for an altered conformation of MoFe protein lacking a FeMoco centre
20. Klebsiella pneumoniae nitrogenase. Formation and stability of putative beryllium fluoride-ADP transition state complexes
21. Redox properties and electron paramagnetic resonance spectroscopy of the transition state complex of Azotobacter vinelandii nitrogenase
22. - stabilized nitrogenase complex and its implication for signal transduction
23. The first glimpse of a complex of nitrogenase component proteins by solution X-ray scattering: Conformation of the electron transfer transition state complex of Klebsiella pneumoniae nitrogenase
24. Comparing crystallographic and solution structures of nitrogenase complexes
25. Chemistry and biochemistry
26. New ribose-modified fluorescent analogues of adenine and guanine nucleotides available as substrates of various enzymes
27. Use of fluorescent analogues to investigate conformational changes in the Fe-Protein of Klebsiella pneumoniae
28. 2 cross linking
29. Nitrogenase of Klebsiella pneumoniae. Purification and properties of the component proteins
30. Catalysis of exchange of terminal phosphate groups of ATP and ADP by purified nitrogenase proteins
31. Microbiuret procedure for protein determination
32. Determination of adenine nucleotides by bioluminescence
33. Principles of fluorescence, Pt VIII, fluorescence polarization
34. Reductant-independent ATP hydrolysis catalyzed by homologous nitrogenase proteins from Azotobacter vinelandii and heterologous crosses with Clostridium pasteurianum
35. 31P NMR studies of enzymatic reactions
36. Biochemical genetics of nitrogenase
37. 4 transition-state complex
38. Redox properties and EPR spectroscopy of the P clusters of MoFe protein