An enzymatically active chimeric protein containing the hydrophilic form of NADPH-cytochrome P450 reductase fused to the membrane-binding domain of cytochrome b5

Biochemical and Biophysical Research Communications

Volumn 284, Issue 4, 2001, Pages 937-941

  Gilep, Andrei A ^b   Guryev, Oleg L ^a   Usanov, Sergey A ^b   Estabrook, Ronald W ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b INSTITUTE OF BIOORGANIC CHEMISTRY   (Belarus)

Author keywords

Affinity chromatography; And electron transfer; Chimeric protein; Cytochrome b5; Cytochrome P450; Heterologous expression in E. coli; NADPH cytochrome P450 reductase

Indexed keywords

CHIMERIC PROTEIN; CYTOCHROME B5; CYTOCHROME P450; CYTOCHROME P450 3A4; CYTOCHROME P450 REDUCTASE; FATTY ACID; FLAVOPROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME MECHANISM; HYDROPHILICITY; HYDROPHOBICITY; MEMBRANE BINDING; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; STEROID HYDROXYLATION;

EID: 0034817388     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5075     Document Type: Article

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