Identification of nonessential Helicobacter pylori genes using random mutagenesis and loop amplification

Research in Microbiology

Volumn 152, Issue 8, 2001, Pages 725-734

  Jenks, Peter J ^a   Chevalier, Catherine ^a   Ecobichon, Chantal ^a   Labigne, Agnès ^a  

^a INSTITUT PASTEUR   (France)

Author keywords

Array; Helicobacter pylori; Hybridization; Inverse PCR; Random mutagenesis; Transposon

Indexed keywords

BACTERIAL DNA; CATALASE; CIRCULAR DNA; UREASE; VIRULENCE FACTOR;

ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENETICS; BACTERIAL MUTATION; BACTERIAL VIRULENCE; BACTERIUM MUTANT; CHROMOSOMAL LOCALIZATION; CONTROLLED STUDY; DNA HYBRIDIZATION; FRAMESHIFT MUTATION; GENE AMPLIFICATION; GENE INSERTION; GENE LIBRARY; GENE MAPPING; GENE MUTATION; GENE SEQUENCE; HELICOBACTER PYLORI; MUTAGENESIS; NONHUMAN; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; STRAIN DIFFERENCE; TRANSPOSON;

HELICOBACTER PYLORI;

EID: 0034817055     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0923-2508(01)01253-0     Document Type: Article

References (19)

1. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori
2. Identification of virulence genes of Helicobacter pylori by random insertion mutagenesis
3. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer
4. Expression of Helicobacter pylori urease genes in Escherichia coli grown under nitrogen-limiting conditions
5. A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity
6. Aflagellated mutants of Helicobacter pylori generated by genetic transformation of naturally competent strains using transposon shuttle mutagenesis
7. Studies on transformation of Escherichia coli plasmids
8. Sequencing microbial genomes-what will it do for microbiology?
9. Systematic cloning of the 1590 Orfs of the 26695 genome as a tool for H. pylori (Hp) gene expression and functional analysis
10. Random mutagenesis of the H. pylori genome
11. Cloning of Campylobacter jejuni genes required for leucine biosynthesis, and construction of leu-negative mutant of C. jejuni by shuttle transposon mutagenesis
12. Shuttle cloning and nucleotide sequence of Helicobacter pylori genes responsible for urease activity
13. Gene disruption and replacement as a feasible approach for mutagenesis of Campylobacter jejuni
14. Genetic and functional characterization of the alpAB gene locus essential for the adhesion of Helicobacter pylori to human gastric tissue
15. Cloning and genetic characterization of Helicobacter pylori catalase and construction of a catalase-deficient mutant strain
16. Shuttle mutagenesis: A method of transposon mutagenesis for Saccharomyces cerevisae
17. The complete genome sequence of the gastric pathogen Helicobacter pylori
18. Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker