Identification of a novel phosphorylation site in human androgen receptor by mass spectrometry

Biochemical and Biophysical Research Communications

Volumn 284, Issue 3, 2001, Pages 836-844

  Zhu, Zixin ^a   Becklin, Robert R ^b   Desiderio, Dominic M ^b   Dalton, James T ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b UNIVERSITY OF TENNESSEE HEALTH SCIENCE CENTER   (United States)

Author keywords

Amino acid sequencing; HPLC coupled electrospray ionization; Human androgen receptor; Matrix assisted laser desorption ionization; Phosphorylation; Tryptic peptide mapping

Indexed keywords

AMINO ACID; ANDROGEN RECEPTOR; ANDROSTANOLONE; HEXAHISTIDINE; HISTIDINE; LIGAND; PEPTIDE; SERINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; DESORPTION; GENE OVEREXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIGAND BINDING; MASS SPECTROMETRY; PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS;

EID: 0034811237     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5030     Document Type: Article

References (29)

1. Gene regulation by steroid hormones
2. The steroid and thyroid hormone receptor superfamily
3. Hormone-induced dissociation of the androgen receptor-heat-shock protein complex: Use of a new monoclonal antibody to distinguish transformed from nontransformed receptors
4. Anti-androgens and the mutated androgen receptor of LNCaP cells: Differential effects on binding affinity, heat-shock protein interaction, and transcription activation
5. Cloning and characterization of a specific coactivator. ARA70, for the androgen receptor in human prostate cells
6. Androgen receptor: An overview
7. Hormonal regulation and identification of chicken progesterone receptor phosphorylation sites
8. Identification of phosphorylated sites in the mouse glucocorticoid receptor
9. Phosphorylation of steroid hormone receptors
10. Dopaminergic and ligand-independent activation of steroid hormone receptors
11. Modulation of the ligand-independent activation of the human estrogen receptor by hormone and antihormone
12. Activation of the human androgen receptor through a protein kinase A signaling pathway
13. Functional domains of the human androgen receptor
14. Steroid requirement for androgen receptor dimerization and DNA binding. Modulation by intramolecular interactions between the NH2-terminal and steroid-binding domains
15. Expression of recombinant androgen receptor in cultured mammalian cells
16. The human androgen receptor: Complementary deoxyribonucleic acid cloning, sequence analysis and gene expression in prostate
17. Structural analysis of complementary DNA and amino acid sequences of human and rat androgen receptors
18. Steroid receptor family: Structure and functions
19. Hormone-dependent phosphorylation of the glucocorticoid receptor occurs mainly in the aminoterminal transactivatiSn domain
20. Forskolin-induced dephosphorylation of the androgen receptor impairs ligand binding
21. Hormone-dependent androgen receptor phosphorylation is accompanied by receptor transformation in human lymph node carcinoma of the prostate cells
22. Identification of three proline-directed phosphorylation sites in the human androgen receptor
23. Phosphorylation on tyrosine of in vitro synthesized human estrogen receptor activates its hormone binding
24. Retinoic acid receptor-beta: Immunodetection and phosphorylation on tyrosine residues
25. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels
26. Identification of gel-separated proteins by liquid chromatography-electrospray tandem mass spectrometry: Comparison of methods and their limitations
27. Identification of a novel proline-directed serine/threonine protein kinase in rat pheochromocytoma
28. Characterization of normal and point-mutated human androgen receptors expressed in the baculovirus system
29. Domains of the human androgen receptor involved in steroid binding, transcriptional activation, and subcellular localization