Time-resolved sperm responses to an egg peptide measured by stopped-flow fluorometry

Biochemical and Biophysical Research Communications

Volumn 284, Issue 2, 2001, Pages 531-535

  Nishigaki, Takuya ^a   Zamudio, Fernando Z ^a   Possani, Lourival D ^a   Darszon, Alberto ^a  

^a INSTITUTO DE BIOTECNOLOGÍA   (Mexico)

Author keywords

Chemotaxis; Egg peptide; Fluorescence quenching; Intracellular Ca2+; Intracellular pH; Ligand receptor interaction; Sperm activation; Stopped flow

Indexed keywords

SPERM ACTIVATING PEPTIDE;

ARTICLE; CALCIUM CELL LEVEL; CELL PH; CONTROLLED STUDY; FLUOROMETRY; NONHUMAN; PRIORITY JOURNAL; RECEPTOR AFFINITY; SEA URCHIN; SIGNAL TRANSDUCTION; SPERM;

EID: 0034809868     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.5000     Document Type: Article

References (24)

1. Structure, function and biosynthesis of sperm-activating peptides and fucose sulfate glycoconjugate in the extracellular coat of sea urchin eggs
2. Chemotaxis of Arbacia punctulata spermatozoa to resact, a peptide from the egg jelly layer
3. Stimulation of sperm respiration rates by speract and resact at alkaline extracellular pH
4. Real-time measurements of the interactions between fluorescent speract and its sperm receptor
5. The participation of speract in the acrosome reaction of Hemicentrotus pulcherrimus
6. Structure and function of asterosaps, sperm-activating peptides from the jelly coat of starfish eggs
7. Purification and the primary structure of sperm-activity peptides from the jelly coat of sea urchin eggs
8. The amino acid sequence and chemical synthesis of speract and of speract analogues
9. Ion channels in sperm physiology
10. Identification and partial characterization of the receptor for speract
11. Speract receptors are localized on sea urchin sperm flagella using a fluorescent peptide analog
12. + channels by the egg peptide speract
13. + channel activated by speract
14. + channel modulated directly by cGMP in the speract-induced signaling cascade of Strongylocentrotus purpuratus sea urchin sperm
15. + exchange in sea urchin spermatozoa through membrane potential changes induced by the egg peptide speract
16. Membrane potential regulates sea urchin sperm adenylylcyclase
17. +-selective channel from the sea urchin sperm plasma membrane
18. Molecular identification of a hyperpolarization-activated channel in sea urchin sperm
19. + entry and increased intracellular pH
20. i increase
21. Effects of sperm-activating peptide I on Hemicentrotus pulcherrimus spermatozoa in high potassium sea water
22. Apparatus for rapid and sensitive spectrophotometry
23. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores