Structural characterization of the 60-kDa Bermuda grass pollen isoallergens, a covalent flavoprotein

Biochemical and Biophysical Research Communications

Volumn 280, Issue 3, 2001, Pages 738-743

  Liaw, Shwu Huey ^a,b   Lee, Donna Y ^b   Chow, Liu Ping ^c   Lau, Gai Xuong ^d   Su, Song Nan ^d  

^a NATIONAL YANG MING UNIVERSITY   (Taiwan)

^b Institute of Molecular Medicine   (Taiwan)

^c NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^d TAIPEI VETERANS GENERAL HOSPITAL   (Taiwan)

Author keywords

Berberine bridge enzyme; Bermuda grass allergen BG60; Domain structure; Flavoprotein; Structural stabilization

Indexed keywords

ALLERGEN; BERBERINE; FLAVINE ADENINE NUCLEOTIDE; FLAVOPROTEIN; ISOPROTEIN; PROTEINASE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; COVALENT BOND; GRASS; MELTING POINT; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN MOTIF; PROTEIN STRUCTURE; SEQUENCE HOMOLOGY; SPECTROSCOPY;

EID: 0034809461     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2000.4203     Document Type: Article

References (14)

1. Identification of allergens and antigens of Bermuda grass (Cynodon dactylon) pollen by immunoblot analysis
2. Isolation and partial characterization of Bermuda grass-pollen allergen: BG-60b
3. Isolation and partial characterization of Bermuda grass pollen allergen, BG-60a
4. Immunologic and physicochemical studies of Bermuda grass pollen antigen BG60
5. The carbohydrate moiety of the Bermuda grass antigen BG60. New oligosaccharides of plant origin
6. Crystallization and preliminary diffraction data of 60-kDa glycosylated pollen isoallergens from Bermuda grass
7. Measuring and increasing protein stability
8. Purification and properties of (S)-tetrahydroprotoberberine oxidase from suspension-cultured cells of Berberis wilsoniae
9. Characterization and mechanism of the berberine bridge enzyme, a covalently flavinylated oxidase of benzophenanthridine alkaloid biosynthesis in plants
10. Identification of the naturally occurring flavin of nitroalkane oxidase from Fusarium oxysporum as a 5-nitrobutyl-FAD and conversion of the enzyme to the active FAD-containing form
11. Covalent attachment of flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) to enzymes: The current state of affairs
12. A putative FAD-binding domain in a distinct group of oxidases including a protein involved in plant development
13. Molecular cloning and characterization of a birch pollen minor allergen, Bet v 5, belonging to a family of isoflavone reductase-related proteins
14. Molecular cloning of major and minor allergens of Alternaria alternata and Cladosporium herbarum