Analyses of the pore forming ability of Bacillus thuringiensis Cry1A mutant toxins using a light-scattering technique

Pesticide Biochemistry and Physiology

Volumn 70, Issue 1, 2001, Pages 7-18

  Daniel, Anu ^a   Dean, Donald H ^c   Adang, Michael J ^a,b  

^a Department of Biochemistry and Molecular Biology ^*  (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c OHIO STATE UNIVERSITY   (United States)

Author keywords

Bacillus thuringiensis; BBMV; Light scattering technique; Manduca sexta; Pore formation

Indexed keywords

BACILLUS THURINGIENSIS; MANDUCA SEXTA;

EID: 0034808285     PISSN: 00483575     EISSN: None     Source Type: Journal    
DOI: 10.1006/pest.2001.2542     Document Type: Article

References (44)

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2. Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins
3. The mode of action of Bacillus thuringiensis delta-endotoxins
4. The crystal δ-endotoxins of Bacillus thuringiensis: Models for their mechanisms of action on the insect gut
5. Dissolution and degradation of Bacillus thuringiensis delta-endotoxin by gut juice protease of the silkworm, Bombyx mori
6. Binding of the delta-endotoxin from Bacillus thuringiensis to brush-border membrane vesicles of the cabbage butterfly (Pieris brassicae)
7. Bacillus thuringiensis δ-endotoxin and larval Manduca sexta midgut brush-border membrane vesicles act synergistically to cause very large increases in the conductance of planar lipid bilayers
8. Restriction of intramolecular movements within the Cry1Aa toxin molecule of Bacillus thuringiensis through disulfide bond engineering
9. Aggregation of Bacillus thuringiensis Cry1A toxins upon binding to target insect larval midgut vesicles
10. Lepidopteran-specific crystal toxins from Bacillus thuringiensis form cation-and anion-selective channels in planar lipid bilayers
11. Colloid-osmotic lysis is a general feature of the mechanism of action of Bacillus thuringiensis delta-endotoxins with different insect specificities
12. Crystal structure of insecticidal δ-endotoxin from Bacillus thuringiensis at 2.5A resolution
13. Bacillus thuringiensis Cry1A(a) insecticidal toxin: Crystal structure and channel formation
14. Site-directed mutations in a highly conserved region of Bacillus thuringiensis δ-endotoxin affect inhibition of short circuit current across Bombyx mori midguts
15. Different domains of Bacillus thuringiensis δ-endotoxins can bind to insect midgut membrane proteins on ligand blots
16. N-Acetylgalactosamine on the putative insect receptor aminopeptidase N is recognized by a site on the domain III lectin-like fold of a Bacillus thuringiensis insecticidal toxin
17. Binding of Bacillus thuringiensis Cry1Ac toxin to Manduca sexta aminopeptidase-N receptor is not directly related to toxicity
18. Domain III of the Bacillus thuringiensis delta-endotoxin Cry1Ac is involved in binding to Manduca sexta brush border membranes and to its purified aminopeptidase N
19. Localized mutagenesis defines regions of the Bacillus thuringiensis delta-endotoxin involved in toxicity and specificity
20. Substitution of residues on the proximal side of Cry1A Bacillus thuringiensis δ-endotoxins affects irreversible binding to Manduca sexta midgut membrane
21. Single amino acid changes in domain II of Bacillus thuringiensis Cry1Ab δ-endotoxin affect irreversible binding to Manduca sexta midgut membrane vesicles
22. Role of domain II, loop 2 residues of Bacillus thuringiensis Cry1Ab δ-endotoxin in reversible and irreversible binding to Manduca sexta and Heliothis virescens
23. Mechanism of inhibition of active potassium transport in isolated midgut of Manduca sexta by Bacillus thuringiensis endotoxin
24. +-gradient-dependent amino acid transport across the brush border membrane of Pieris brassicae midgut cells
25. Effects of Bacillus thuringiensis delta-endotoxin on the permeability of brush border membrane vesicles from tobacco hornworm (Manduca sexta) midgut
26. Barium and calcium block Bacillus thuringiensis subspecies kurstaki delta-endotoxin inhibition of potassium current across isolated midgut of larval Manduca sexta
27. δ-endotoxins induce cation channels in Spodoptera frugiperda brush border membranes in suspension and in planar lipid bilayers
28. An analysis of Bacillus thuringiensis δ-endotoxin action on insect-midgut-membrane permeability using a light-scattering assay
29. Site-directed mutations in the third domain of Bacillus thuringiensis δ-endotoxin Cry1Aa affect its ability to increase the permeability of Bombyx mori midgut brush border membrane vesicles
30. Comparative analysis of the individual protoxin components in P1 crystals of Bacillus thuringiensis subsp. kurstaki isolates NRD-12 and HD-1
31. Mutations in domain I of Bacillus thuringiensis δ-endotoxin Cry1Ab reduce the irreversible binding of toxin to Manduca sexta brush border membrane vesicles
32. Role of two arginine residues in domain II, loop 2 of Cry1Ab and Cry1Ac Bacillus thuringiensis δ-endotoxin in toxicity and binding to Manduca sexta and Lymantria dispar aminopeptidase N
33. Removal of adsorbed toxin fragments that modify Bacillus thuringiensis Cry1C δ-endotoxin iodination and binding by sodium dodecyl sulfate treatment and renaturation
34. Location of a Bombyx mori receptor binding region on a Bacillus thuringiensis delta-endotoxin
35. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
36. Revision of two computer programs for probit analysis
37. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
38. Preparation and partial characterization of amino acid transporting brush border membrane vesicles from the larval midgut of the cabbage butterfly (Pieris brassicae)
39. Resistance to the Bacillus thuringiensis bioinsecticide in a field population of Plutella xylostella is due to a change in a midgut membrane receptor
40. Identification of putative brush border membrane binding proteins specific to Bacillus thuringiensis delta-endotoxin by protein blot analysis
41. Insecticidal crystal proteins of Bacillus thuringiensis
42. A vacuolar-type proton pump in a vesicle fraction enriched with potassium transporting plasma membranes from tobacco hornworm midgut
43. Nystatin as a probe for investigating the electrical properties of a tight epithelium
44. The structure and organization within the membrane of the helices composing the pore-forming domain of Bacillus thuringiensis δ-endotoxin are consistent with an "umbrella-like" structure of the pore