Permeabilization of Pichia pastoris for glycolate oxidase activity

Biotechnology Letters

Volumn 23, Issue 18, 2001, Pages 1535-1537

  Gough, S ^a   Deshpande, M ^a   Scher, M ^a   Rosazza, J P N ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

Glycolate oxidase; Permeabilization; Pichia pastoris; Yeast

Indexed keywords

BENZALKONIUM CHLORIDE; HYDROXY ACID OXIDASE A;

ARTICLE; CATALYSIS; CELL MEMBRANE PERMEABILITY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; INCUBATION TIME; NONHUMAN; PICHIA PASTORIS; TEMPERATURE;

EID: 0034807449     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1011601827503     Document Type: Article

References (6)

1. Dependence of yeast permeabilization with Triton X-100 on the cell age
2. Pyruvic acid production using methylotropic yeast transformants
3. Permeabilized cells
4. Permeabilization of Bakers' yeast by cetyltrimethylammonium bromide for intracellular enzyme catalysis
5. A rapid permeabilization procedure for accurate quantitative determination of β-galactosidase activity in yeast cells
6. Permeabilization of Kluyveromyces lactis cells for milk whey saccharification: A comparsion of different treatments