Structural investigations of the active-site mutant Asn156A1a of outer membrane phospholipase A: Function of the Asn-His interaction in the catalytic triad

Protein Science

Volumn 10, Issue 10, 2001, Pages 1962-1969

  Snijder, H J ^a   Van Eerde, J H ^a   Kingma, R L ^b   Kalk, K H ^a   Dekker, N ^b,c   Egmond, M R ^b   Dijkstra, B W ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UTRECHT UNIVERSITY   (Netherlands)

^c ASTRAZENECA R AND D   (Sweden)

Author keywords

Active site mutant; Catalytic triad; Histidine tautomer; Low barrier hydrogen bond; Membrane protein; Phospholipase; Serine hydrolase; X ray crystal structure

Indexed keywords

ALANINE; ASPARAGINE; HISTIDINE; MUTANT PROTEIN; OUTER MEMBRANE PROTEIN; PHOSPHOLIPASE A;

ARTICLE; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME STABILITY; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROGEN BOND; NONHUMAN; OUTER MEMBRANE; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; STRUCTURE ANALYSIS; THERMOSTABILITY; X RAY CRYSTALLOGRAPHY;

ALANINE; AMINO ACID SUBSTITUTION; ASPARAGINE; BACTERIAL OUTER MEMBRANE PROTEINS; BINDING SITES; ESCHERICHIA COLI; HEAT; HISTIDINE; HYDROGEN BONDING; HYDROGEN-ION CONCENTRATION; MODELS, MOLECULAR; PHOSPHOLIPASES A; PROTEIN CONFORMATION; PROTEIN DENATURATION;

EID: 0034802508     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.17701     Document Type: Article

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