Swapping of functional domains using a chimeric monster deletion mutagenesis strategy

BioTechniques

Volumn 31, Issue 4, 2001, Pages 724-728

  Yang, C H ^a   Zwieb, C ^a  

^a UNIVERSITY OF TEXAS HEALTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA FRAGMENT; SIGNAL RECOGNITION PARTICLE;

ARTICLE; CHIMERA; DNA TEMPLATE; GENE DELETION; GENE MUTATION; HUMAN; METHANOCOCCUS JANNASCHII; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

EID: 0034800291     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01314bm02     Document Type: Article

References (9)

1. Assembly of archaeal signal recognition particle from recombinant components
2. Systematic site-directed mutagenesis of protein SRP19: Identification of the residues essential for binding to signal recognition particle RNA
3. Engineering hybrid genes without the use of restriction enzymes: Gene splicing by overlap extension
4. Isolation and characterization of a cDNA clone encoding the 19 kDa protein of signal recognition particle (SRP): Expression and binding to 7SL RNA
5. A general method of site-specific mutagenesis using a modification of the Thermus aquaticus polymerase
6. Prevention of unexpectedly long PCR products primed at short inverted repeats
7. PCR-assisted mutagenesis for site-directed insertion/deletion of large DNA segments
8. Interaction of protein SRP19 with signal recognition particle RNA lacking individual RNA helices
9. Recognition of a tetranucleotide loop of signal recognition particle RNA by protein SRP19