Sensitive and specific detection of Pseudomonas avellanae using primers based on 16S rRNA gene sequences

Journal of Phytopathology

Volumn 149, Issue 9, 2001, Pages 527-532

  Scortichini, M ^a   Marchesi, U ^a  

^a Istituto Sperimentale per la Frutticoltura   (Italy)

Author keywords

16S rRNA gene; Detection; Hazelnut decline; Primers; Pseudomonas avellanae

Indexed keywords

BACTERIAL GENE; BACTERIAL INFECTION; BACTERIUM DETECTION; GENETIC SCREENING; GENETIC STRAIN; GREECE; ITALY; PATHOVAR; POLYMERASE CHAIN REACTION; PRIMER RNA; RIBOSOME RNA; RNA 16S; SPECIFICITY;

BACTERIUM; DETECTION METHOD; GENETICS; PATHOGEN;

BACTERIA (MICROORGANISMS); CORYLUS; CORYLUS AVELLANA; PROKARYOTA; PSEUDOMONAS; PSEUDOMONAS AVELLANAE; PSEUDOMONAS SYRINGAE PV. SYRINGAE;

EID: 0034797284     PISSN: 09311785     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1439-0434.2001.00670.x     Document Type: Article

References (11)

1. Attenuation of PCR inhibition in the presence of plant compounds by addition of BLOTTO
2. Reclassification of Pseudomonas syringae pv. syringae as Pseudomonas avellanae (spec. nov.), the bacterium causing canker of hazelnut (Corylus avellana L.)
3. An efficient method for isolation of RNA and DNA from plants containing polyphenolics
4. The determination and comparison of the 16S rRNA gene sequences of species of the genus Pseudomonas (sensu strictu) and estimation of the natural intrageneric relationships
5. The problem of bacterial canker of hazelnut in Greece caused by Pseudomonas syringae pv. syringae
6. Systemic migration of Pseudomona syringae pr. avellanae in twigs and young trees of hazelnut and symptom development
7. Differentiation of Pseudomonas avellanae strains from Greece and Italy by rep-PCR genomic fingerprinting
8. Rapid identification of Pseudomonas avellanae field isolates, causing hazelnut decline in central Italy, by repetitive PCR genomic fingerprinting
9. Genetic and pathogenic diversity of Pseudomonas avellanae strains isolated from Corylus avellana trees in north-west of Italy, and comparison with strains from other regions
10. Occurrence of Pseudomonas avellanae (Psallidas) Janse et at., related pseudomonads on wild Corylus avellana trees and genetic relationships with strains isolated from cultivated hazelnuts
11. Differentiation of Pseudomonas solanacearum, Pseudomonas syzygii, Pseudomonas picketii and the blood disease bacterium by partial 16S rRNA sequencing: Construction of oligonucleotide primers for sensitive detection by polymerase chain reaction