Cloning of deleted sequences (CODE): A genomic subtraction method for enriching and cloning deleted sequences

BioTechniques

Volumn 31, Issue 4, 2001, Pages 788-793

  Li, J ^a   Wang, F ^a   Kashuba, V ^a   Wahlestedt, C ^a   Zabarovsky, E R ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

DISEASE CONTROL; DNA; ENZYMES; GENES; TUMORS;

HYBRIDIZATION;

CLONING;

DNA; THYMIDINE TRIPHOSPHATE; URACIL DNA GLYCOSYLTRANSFERASE;

ARTICLE; CARCINOGENESIS; DNA DENATURATION; DNA HYBRIDIZATION; GENE AMPLIFICATION; GENE DELETION; HUMAN; MOLECULAR CLONING; POLYMERASE CHAIN REACTION;

EID: 0034797258     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01314st05     Document Type: Article

References (10)

1. CpG islands as gene markers in the vertebrate nucleus
2. Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries
3. Sequence analysis of a 685-kb genomic region on chromosome 3p22-p21.3 that is homozygously deleted in a lung carcinoma cell line
4. Cloning of polymorphisms (COP): Enrichment of polymorphic sequences from complex genomes
5. Cloning the differences between two complex genomes
6. Comparative genomic analysis of tumors: Detection of DNA losses and amplification
7. Detecting gene copy number fluctuations in tumor cells by microarrays analysis of genomic representations
8. "RFLP subtraction": A method for making libraries of polymorphic markers
9. Isolation of differentially expressed genes upon immunoglobulin class switching by a subtractive hybridization method using uracil DNA glycosylase
10. CIS-cloning of identical sequences between two complex genomes