Elastin expression in cultured human keratinocytes: Exon 26A of elastin primary transcript is always included in terminally differentiated keratinocytes

Archives of Dermatological Research

Volumn 293, Issue 8, 2001, Pages 430-433

  Hirano, Eiichi ^a   Okamoto, Kouji ^b   Matsubara, Yuko ^c   Takehana, Makoto ^c   Kobayashi, Shizuko ^c   Tajima, S ^a  

^a NATIONAL DEFENSE MEDICAL COLLEGE   (Japan)

^b KYUSHU INSTITUTE OF TECHNOLOGY   (Japan)

^c KEIO UNIVERSITY   (Japan)

Author keywords

Alternative splicing; Elastin; Keratinocyte; Terminal differentiation

Indexed keywords

COLLAGEN; DNA FRAGMENT; ELASTIN; FIBRONECTIN;

AMINO ACID SEQUENCE; ARTICLE; CELL CULTURE; CONNECTIVE TISSUE; CONTROLLED STUDY; EXON; FIBROBLAST; GENETIC CODE; GENETIC TRANSCRIPTION; HUMAN; HUMAN TISSUE; KERATINOCYTE; NORMAL HUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING; SKIN;

ALTERNATIVE SPLICING; CELL DIFFERENTIATION; CELLS, CULTURED; ELASTIN; EXONS; FIBROBLASTS; HUMANS; KERATINOCYTES; RNA, MESSENGER; SKIN;

EID: 0034791847     PISSN: 03403696     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004030100247     Document Type: Article

References (22)

1. Alternative splicing in the control of gene expression
2. Alternate exon usage is a commonly used mechanism for increasing coding diversity within genes coding for extra-cellular matrix proteins
3. Alternative splicing of human elastin mRNA indicated by sequence analysis of cloned genomic and complementary DNA
4. Sequence variation of bovine elastin mRNA due to alternative splicing
5. Structure of the bovine elastin gene and S1 nuclease analysis of alternative splicing of elastin mRNA in the bovine nuchal ligament
6. Cloning of full-length elastin cDNAs from a human skin fibroblast recombinant cDNA library: Further elucidation of alternative splicing using exon-specific probes
7. Quantitation of tropoelastin mRNA and assessment of alternative splicing in human skin fibroblasts by reverse transcriptase-polymerase chain reaction
8. Structure of the elastin gene
9. Cultured human keratinocytes express tropoelastin
10. Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentration in vitro
11. Fibronectin inhibits the terminal differentiation of human keratinocytes
12. Expression of type XVI collagen in cultured skin fibroblasts is related to cell growth arrest
13. Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
14. Amplification of RNA
15. A method for nested PCR with single closed reaction tube
16. DNA sequencing with chain-terminating inhibitors
17. Oxidation, cross-linking and insolubilization of recombinant tropoelastin by purified lysyl oxidase
18. Prediction of the secondary structure and antigenicity of human and bovine tropoelastins
19. The amino acid sequence coded by the rarely expressed exon 26A of human elastin contains a stable β-turn with chemotactic activity for monocytes
20. An open reading frame element mediates posttranscriptional regulation of tropoelastin and responsiveness to transforming growth factor beta 1
21. Mammalian tropoelastin: Multiple domains of the protein define an evolutionarily divergent amino acid sequence
22. Alternative splicing of rat tropoelastin mRNA is tissue specific and developmentally regulated