Analysis of the S2 subsite specificities of the recombinant cysteine proteinases CPB of Leishmania mexicana, and cruzain of Trypanosoma cruzi, using fluorescent substrates containing non-natural basic amino acids

Molecular and Biochemical Parasitology

Volumn 117, Issue 2, 2001, Pages 137-143

  Alves, Lira C ^a   Melo, Robson L ^a   Cezari, Maria Helena S ^a   Sanderson, Sanya J ^b   Mottram, Jeremy C ^c   Coombs, Graham H ^b   Juliano, Luiz ^a   Juliano, Maria A ^a  

^a FEDERAL UNIVERSITY OF SÃO PAULO   (Brazil)

^b UNIVERSITY OF GLASGOW   (United Kingdom)

^c UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Cathepsin B; Cathepsin L; Cruzain; Cruzipain; Cysteine proteinase of Leishmania mexicana; Papain

Indexed keywords

3 PYRIDYLALANINE; 4 AMINOCYCLOHEXYLALANINE; 4 AMINOMETHYL N ISOPROPYLPHENYLALANINE; 4 AMINOMETHYLCYCLOHEXYLALANINE; 4 AMINOMETHYLPHENYLALANINE; 4 GUANIDINE PHENYLALANINE; 4 PIPERIDINYLALANINE; ALANINE DERIVATIVE; AMINO ACID; ARGININE; BENZOYLPHENYLALANYLARGINYLMETHIONYLCYSTYLALANINE; CATHEPSIN B; CATHEPSIN L; CRUZIPAIN; CYSTEINE PROTEINASE; ENZYME INHIBITOR; PHENYLALANINE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; HYDROLYSIS; HYDROPHOBICITY; LEISHMANIA MEXICANA; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; TRYPANOSOMA CRUZI;

AMINO ACIDS, BASIC; ANIMALS; CATALYTIC DOMAIN; CATHEPSIN B; CATHEPSINS; CYSTEINE ENDOPEPTIDASES; FLUORESCENCE; HYDROLYSIS; LEISHMANIA MEXICANA; PEPTIDES; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY; TRYPANOSOMA CRUZI;

LEISHMANIA MEXICANA; TRYPANOSOMA; TRYPANOSOMA CRUZI;

EID: 0034788639     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(01)00340-1     Document Type: Article

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