Replacement of arginine-171 and aspartate-453 in Streptomyces coelicolor malate synthase A by site-directed mutagenesis inactivates the enzyme

Applied Microbiology and Biotechnology

Volumn 57, Issue 3, 2001, Pages 363-367

  Goh, L L ^a   Loke, P ^a   Sim, T S ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYL COENZYME A; ARGININE; ASPARTIC ACID; MAGNESIUM ION; MALATE SYNTHASE; MALATE SYNTHASE A; MUTANT PROTEIN; UNCLASSIFIED DRUG;

BIOTECHNOLOGY; ENZYME;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME INACTIVATION; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; STREPTOMYCES COELICOLOR;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARGININE; ASPARTIC ACID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ESCHERICHIA COLI; MALATE SYNTHASE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE HOMOLOGY, AMINO ACID; STREPTOMYCES;

ACTINOBACTERIA (CLASS); ESCHERICHIA COLI; NEGIBACTERIA; STREPTOMYCES COELICOLOR; UNCULTURED ACTINOMYCETE;

EID: 0034787623     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530100785     Document Type: Article

References (11)

1. Malate synthase from Streptomyces clavuligerus NRRL 3585: Cloning, molecular characterization and its control by acetate
2. Crystal structure of Escherichia coli malate synthase G complexed with magnesium and glyoxylate at 2.0 Å resolution: Mechanism implications
3. Gene cloning and sequencing, and enzyme purification of the malate synthase of Streptomyces arenae
4. The role and control of the glyoxylate cycle in Escherichia coli
5. Molecular cloning, heterologous expression and functional characterization of a malate synthase gene from Streptomyces coelicolor A3(2)
6. Strategies for achieving high-level expression of genes inEscherichia coli
7. Elevated levels of glyoxylate shunt enzymes in Escherichia coli strains constitutive for fatty acid degradation
8. Molecular characterization of Escherichia coli malate synthase G: Differentiation with the malate synthase A isozyme
9. Regulation of glyoxylate metabolism in Escherichia coli K-12
10. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice
11. Active site residue 297 of Aspergillus niger phytase critically affects the catalytic properties