Cloning, expression, purification and characterization of patatin, a novel phospholipase A

European Journal of Biochemistry

Volumn 268, Issue 19, 2001, Pages 5037-5044

  Hirschberg, Hoang J H B ^a   Simons, Jan Willem F A ^a   Dekker, Niek ^a   Egmond, Maarten R ^a,b  

^a UTRECHT UNIVERSITY   (Netherlands)

^b Department of Enzymology and Protein Engineering ^*  (Netherlands)

Author keywords

Active site dyad; Characterization; Patatin cloning; Phospholipase A

Indexed keywords

1,2 DINONANOYLGLYCERO 3 PHOSPHOCHOLINE; 4 NITROPHENYLESTER; ASPARTIC ACID; CARBON; DIOCTANOYLLECITHIN; ENZYME; ESTER DERIVATIVE; ESTERASE; GLYCOPROTEIN; GLYCOSYLATED PROTEIN; HISTIDINE; PATATIN; PHOSPHOLIPASE A; PHOSPHOLIPASE A2; PHOSPHORYLCHOLINE; RECOMBINANT PROTEIN; SERINE; SIGNAL PEPTIDE; TRITON X 100; UNCLASSIFIED DRUG; UREA;

ARTICLE; CELL INCLUSION; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE OVEREXPRESSION; MOLECULAR CLONING; MULTIGENE FAMILY; NONHUMAN; POTATO; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN ISOLATION; SEQUENCE HOMOLOGY; SEQUENCE TAGGED SITE; SOLUBILIZATION; WEIGHT;

AMINO ACID SEQUENCE; BASE SEQUENCE; BINDING SITES; CARBOXYLIC ESTER HYDROLASES; CHROMATOGRAPHY, GEL; CLONING, MOLECULAR; CYTOSOL; DNA PRIMERS; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PHOSPHOLIPASES; PLANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; SOLANUM TUBEROSUM;

EID: 0034787011     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.0014-2956.2001.02411.x     Document Type: Article

References (18)

1. 2 reveals a novel topology and catalytic mechanism
2. Lipid acyl hydrolase of patatin
3. Characterization of the lipid acyl hydrolase activity of the major potato (Solanum tuberosum) tuber protein, patatin, by cloning and abundant expression in a baculovirus vector
4. Expression of a tuber specific storage protein in transgenic tobacco plants; demonstration of an esterase activity
5. Targeting and glycosylation of patatin the major potato tuber protein in leaves of transgenic tobacco
6. Expression of mutant patatin protein in transgenic tobacco plants: Role of glycans and intracellular location
7. Esterase isozymes from solanum tubers
8. 2-terminal sequence analysis
9. Molecular cloning and analysis of four potato tuber mRNAs
10. Genetic and physical mapping of the patatin genes in potato and tomato
11. Structural diversity of the patatin gene family in potato cv
12. The two classes of genes for the major potato tuber protein, patatin, are differentially expressed in tubers and roots
13. The enzymic breakdown of lipids in potato tuber by phospholipid- and galactolipid-acyl hydrolase activities and by lipoxygenase
14. The enzymic deacylation of phospholipids and galactolipids in plants
15. A cytosolic phospholipase A2 from potato tissues appears to be patatin
16. Application of potato lipid acyl hydrolase for the synthesis of monoacylglycerols
17. Esterase specificity of patatin from two potato cultivars
18. Role of essential histidine residues in L-α-hydroxy acid oxidase from rat kidney