Phalloidin-eosin followed by photo-oxidation: A novel method for localizing F-actin at the light and electron microscopic levels

Journal of Histochemistry and Cytochemistry

Volumn 49, Issue 11, 2001, Pages 1351-1361

  Capani, F ^a   Deerinck, T J ^a   Ellisman, M H ^a   Bushong, E ^a   Bobik, M ^a   Martone, M E ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

3D reconstruction; Cytoskeleton; Dendritic spines; Electron tomography; Purkinje cells

Indexed keywords

DIAMINOBENZIDINE; EOSIN; F ACTIN; GLUTARALDEHYDE; OSMIUM TETRAOXIDE; PHALLOIDIN;

ANALYTIC METHOD; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY LABELING; ARTICLE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DENDRITIC SPINE; ELECTRON MICROSCOPY; MALE; MICROSCOPY; NONHUMAN; PH; PHOTOOXIDATION; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN LOCALIZATION; RAT; TISSUE FIXATION;

EID: 0034783185     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540104901103     Document Type: Article

References (21)

1. Contractile units in stress fibers of fetal human astroglia in tissue culture
2. Selective localization of high concentrations of F-actin in subpopulations of dendritic spines in rat central nervous system: A 3-dimensional electron microscopic study
3. Immunochemical localization of actin in dendritic spines of the cerebral cortex using colloidal gold as a probe
4. Effects of cytochalasin and phalloidin on actin
5. Chemokines induce migration and changes in actin polymerization in adult rat brain microglia and a human fetal microglial cell line in vitro
6. Fluorescence photo-oxidation with eosin: A method for high-resolution immulocalization and in situ hybridization detection for light and electron microscopy
7. Actin in the nervous system
8. Cytoplasmic actin in neuronal processes as a possible mediator of synaptic plasticity
9. Rapid actin-based plasticity in dendritic spines
10. Specific labeling of connexin43 in NRK cells using tyramide-based signal amplification and fluorescence photo-oxidation
11. Studies on the organization and localization of actin and myosin in neurons
12. Cytoplasmic organization in cerebellar dendritic spines
13. Synaptic vesicle populations in saccular hair cells reconstructed by electron tomography
14. Neuronal mapping: A photo-oxidation reaction makes Lucifer Yellow useful for electron microscopy
15. High actin concentrations in brain dendritic spines and postsynaptic densities
16. Actin filament destruction by osmium tetroxide
17. Beta-Actin is confined to structures having high capacity of remodeling in developing and adult rat cerebellum
18. Electron microscopy of actin and myosin
19. Photoconversion of some fluorescent markers to diaminobenzidine product
20. Fluorescent phallotoxin, a tool for the visualization of cellular actin
21. Protein folding in a specialized compartment: The endoplasmic reticulum