The functional unit of interphotoreceptor retinoid-binding protein (IRBP) - Purification, characterization and preliminary crystallographic analysis

Experimental Eye Research

Volumn 73, Issue 2, 2001, Pages 257-264

  Loew, Andreas ^a   Baer, Claxton ^b   Gonzalez Fernandez, Federico ^b  

^a UNIVERSITY OF TEXAS AT DALLAS   (United States)

^b UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

Crystal structure; Interphotoreceptor matrix; Interphotoreceptor retinoid binding protein; IRBP; Retina

Indexed keywords

HYBRID PROTEIN; INTERPHOTORECEPTOR RETINOID BINDING PROTEIN; RETINOL; SELENOMETHIONINE; THIOREDOXIN;

ARTICLE; CRYSTAL STRUCTURE; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN PURIFICATION; X RAY CRYSTALLOGRAPHY; XENOPUS LAEVIS;

EID: 0034775371     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1006/exer.2001.1031     Document Type: Article

References (36)

1. Human interphotoreceptor matrix contains serum albumin and retinol-binding protein
2. Size and shape of bovine interphotoreceptor retinoid-binding protein by electron microscopy and hydrodynamic analysis
3. Expression and characterization of the fourth repeat of Xenopus interphotoreceptor retinoid-binding protein in E. coli
4. Soluble expression in E. coli of a functional interphtoreceptor retinoid-binding protein module fused to thioredoxin: Correlation of vitmain A binding regions with conserved domains of C-terminal processing proteases
5. Truncational mutagenesis of the fourth module of Xenopus IRBP
6. Endogenous fatty acids are covalently and noncovalently bound to interphotoreceptor retinoid-binding protein in the monkey retina
7. The retinal pigment epithelium: A versatile partner in vision
8. Binding affinities of retinol and related compounds to retinol binding proteins
9. Retinoids and the visual process
10. Preparation of selenomethionyl proteins for phase determination
11. Fourth module of Xenopus interphotoreceptor retinoid-binding protein: Activity in retinoid transfer between the retinal pigment epithelium and rod photoreceptors
12. Early expression of the gene for interphotoreceptor retinol-binding protein during photoreceptor differentiation suggests a critical role for the interphotoreceptor matrix in retinal development
13. Prediction of structural and functional relationships of Repeat 1 of human interphotoreceptor retinoid-binding protein (IRBP) with other proteins
14. Effects of dispersed point substitutions in Repeat 1 of human interphotoreceptor retinoid binding protein (IRBP)
15. Sparse matrix signaling: A screening method for crystallization of proteins
16. Overview of isomorphous replacement phasing
17. Transgene expression in Xenopus rods
18. Early onset photoreceptor abnormalities induced by targeted disruption of the interphotoreceptor retinoid-binding protein gene
19. Crystallization and preliminary crystallographic analysis of the second module of Xenopus interphotoreceptor retinoid binding protein
20. Histidine patch thioredoxins. Mutant forms of thioredoxin with metal chelating affinity that provide for convenient purifications of thioredoxin fusion proteins
21. Solvent content of protein crystals
22. Expression and purification of thioredoxin fusion proteins
23. The interphotoreceptor matrix, a space in sight
24. Structure-function relationships in the four repeats of human interphotoreceptor retinoid-binding protein (IRBP)
25. Molecular studies of CtpA, the carboxyl-terminal processing protease for the D1 protein of the photosystem II reaction center in higher plants
26. Processing of X-ray diffraction data collected in oscillation mode
27. Kinetics of visual pigment regeneration in excised mouse eyes and in mice with a targeted disruption of the gene encoding interphotoreceptor retinoid-binding protein or arrestin
28. Interphotoreceptor retinoid-binding protein (IRBP). Molecular biology and physiological role in the visual cycle of rhodopsin
29. Zebrafish interphotoreceptor retinoid-binding protein: Differential circadian expression among cone subtypes
30. The rhodopsin cycle is preserved in IRBP "knockout" mice despite abnormalities in retinal structure and function
31. Retinoids in photosensitive systems
32. Interphotoreceptor retinoid-binding protein contains three retinoid binding sites
33. The structures of the asparagine-linked sugar chains of bovine interphotoreceptor retinol-binding protein. Occurrence of fucosylated hybrid-type oligosaccharides
34. Goldfish cones secrete a two-repeat interphotoreceptor retinoid-binding protein
35. Measurement of ligand binding to protein by fluorescence spectroscopy
36. Increase of solubility of foreign proteins in Escherichia coli by coproduction of the bacterial thioredoxin