Brefeldin A rapidly disrupts plasma membrane polarity by blocking polar sorting in common endosomes of MDCK cells

Journal of Cell Science

Volumn 114, Issue 18, 2001, Pages 3309-3321

  Wang, E ^a   Pennington, J G ^a   Goldenring, J R ^a   Hunziker, W ^a   Dunn, K W ^a  

^a INDIANA UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

Endocytosis; Epithelium; MDCK cell; Polarity; Transferrin

Indexed keywords

BREFELDIN A; GREEN FLUORESCENT PROTEIN; LOW DENSITY LIPOPROTEIN; RAB PROTEIN; TRANSFERRIN; TRANSFERRIN RECEPTOR;

ANIMAL CELL; APICAL MEMBRANE; ARTICLE; BASOLATERAL MEMBRANE; CELL COMPARTMENTALIZATION; CELL MEMBRANE; CELL PH; CELL POLARITY; CELL STRAIN; CELL VACUOLE; CHIMERA; CONTROLLED STUDY; DISSOCIATION; ENDOCYTOSIS; ENDOSOME; NONHUMAN; PRIORITY JOURNAL; PROTEIN TARGETING; PROTEIN TRANSPORT; TIGHT JUNCTION; TRANSCYTOSIS;

ADAPTOR PROTEIN COMPLEX GAMMA SUBUNITS; ANIMALS; BIOLOGICAL TRANSPORT; BREFELDIN A; CELL COMPARTMENTATION; CELL MEMBRANE; CELL POLARITY; CELLS, CULTURED; ENDOSOMES; HUMANS; IMMUNOGLOBULIN A; MEMBRANE PROTEINS; MICE; PROTEIN TRANSPORT; RAB GTP-BINDING PROTEINS; RABBITS; RECEPTORS, TRANSFERRIN; TRANSFERRIN;

ANIMALIA;

EID: 0034773182     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (30)

1. Receptor-mediated transcytosis of IgA in MDCK cells is via apical recycling endosomes
2. Basolateral to apical transcytosis in polarized cells is indirect and involves BFA and trimeric G protein sensitive passage through the apical endosome
3. Endocytosis in filter-grown Madin-Darby canine kidney cells
4. Expression and analysis of the polymeric immunoglobulin receptor in Madin-Darby canine kidney cells using retroviral vectors
5. Definition of distinct compartments in polarized Madin-Darby canine kidney (MDCK) cells for membrane-volume sorting, polarized sorting and apical recycling
6. Association of Rab25 and Rab11a with the apical recycling system of polarized Madin-Darby canine kidney cells
7. Brefeldin A inhibits Golgi membrane-catalysed exchange of guanine nucleotide onto ARF protein
8. A novel clathrin adaptor complex mediates basolateral targeting in polarized epithelial cells
9. In polarized MDCK cells basolateral vesicles arise from clathrin-gamma-adaptin-coated domains on endosomal tubules
10. Selective inhibition of transcytosis by brefeldin A in MDCK cells
11. Rab17 localizes to recycling endosomes and regulates receptor-mediated transcytosis in epithelial cells
12. Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic
13. Common signals control low density lipoprotein receptor sorting in endosomes and the Golgi complex of MDCK cells
14. Studies of endocytosis using image intensification fluorescence microscopy and digital image analysis
15. Apical and basolateral endosomes of MDCK cells are interconnected and contain a polarized sorting mechanism
16. Mu1B, a novel adaptor medium chain expressed in polarized epithelial cells
17. Identification of clathrin and clathrin adaptors on tubulovesicles of gastric acid secretory (oxyntic) cells
18. Interactions of the AP-1 Golgi adaptor with the polymeric immunoglobulin receptor and their possible role in mediating brefeldin A-sensitive basolateral targeting from the trans-Golgi network
19. Meeting of the apical and basolateral endocytic pathways of the Madin-Darby canine kidney cell in late endosomes
20. Acetoacetylated lipoproteins used to distinguish fibroblasts from macrophages in vitro by fluorescence microscopy
21. ATP and cytosol requirements for transferrin recycling in intact and disrupted MDCK cells
22. Effects of brefeldin A on endocytosis, transcytosis and transport to the Golgi complex in polarized MDCK cells
23. A novel class of clathrin-coated vesicles budding from endosomes
24. In AtT20 and HeLa cells brefeldin A induces the fusion of tubular endosomes and changes their distribution and some of their endocytic properties
25. Partial colocalization of glucocorticoid and mineralocorticoid receptors in discrete compartments in nuclei of rat hippocampus neurons
26. Brefeldin A causes structural and functional alterations of the trans-Golgi network of MDCK cells
27. Brefeldin A. enhances receptor-mediated transcytosis of transferrin in filter-grown Madin-Darby canine kidney cells
28. Apical and basolateral endocytic pathways of MDCK cells meet in acidic common endosomes distinct from a nearly-neutral apical recycling endosome
29. Brefeldin A causes a microtubule-mediated fusion of the trans-Golgi network and early endosomes
30. Segregation of transferrin to a mildly acidic (pH 6.5) para-Golgi compartment in the recycling pathway