Toward a biotechnological heparin through combined chemical and enzymatic modification of the Escherichia coli K5 polysaccharide

Seminars in Thrombosis and Hemostasis

Volumn 27, Issue 5, 2001, Pages 437-443

  Naggi, A ^a   Torri, G ^a   Casu, B ^a   Oreste, P ^a   Zoppetti, G ^a   Li, J P ^a   Lindahl, U ^a  

^a Istituto di Ricerche Chimiche e Biochimiche G Ronzoni   (Italy)

Author keywords

Biotechnological heparin; Chemical and enzymatic modification; K5 polysaccharide; Sulfation patterns; Sulfation desulfation

Indexed keywords

BACTERIAL POLYSACCHARIDE; BLOOD CLOTTING FACTOR 10A; BLOOD CLOTTING FACTOR 10A INHIBITOR; EPIMERASE; GLUCURONIC ACID; HEPARAN SULFATE; HEPARIN; HEPARIN DERIVATIVE; HEPARIN PERSULFATE; IDURONIC ACID; N ACETYLHEPAROSAN; PROTEOHEPARAN SULFATE; UNCLASSIFIED DRUG;

BIOTECHNOLOGY; CHEMICAL MODIFICATION; CHEMICAL REACTION KINETICS; DEACETYLATION; DESULFURIZATION; DRUG SULFATION; DRUG SYNTHESIS; ENZYME MECHANISM; ESCHERICHIA COLI; GLYCOSAMINOGLYCAN METABOLISM; HEPARINIZATION; NONHUMAN; POLYMERIZATION; PRIORITY JOURNAL; PROTEIN PROCESSING; REVIEW; SEQUENCE ANALYSIS; STRUCTURE ANALYSIS;

ANIMALS; BIOTECHNOLOGY; ESCHERICHIA COLI; FACTOR XA; HEPARIN; HUMANS; POLYSACCHARIDES, BACTERIAL; SULFATES;

EID: 0034771647     PISSN: 00946176     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-2001-17954     Document Type: Review

References (26)

1. The structure of the capsular polysaccharide (K5 antigen) of urinary-tract-infective Escherichia coli 010:K5:H4
2. Biosynthesis of hepatin. Use of Escherichia coli capsular polysaccharide as a model substrate in enzymic polymer modification reaction
3. Biologically active heparan sulfate-like species by combined chemical and enzymic modification of the Escherichia coli polysaccharide K5
4. Heparin-like compounds prepared by chemical modification of capsular polysaccharide from E. coli K5
5. Structural and functional properties of heparin analogues obtained by chemical sulfation of Escherichia coli capsular polysaccharide
6. Structure and biological interactions of heparin and heparan sulfate
7. The EXT1/EXT2 tumor suppressors: Catalytic activities and role in heparan sulfate biosynthesis
8. Regulated diversity of heparan sulfate
9. Proteoglycans: Structures and interactions
10. Production of K5 polysaccharides of different molecular weight by Escherichia coli
11. 35S] sulfamate derivatives for studies on heparin degrading enzymes of mammalian origin
12. Biosynthesis of heparin/heparan sulfate: Mechanism of epimerization of glucuronosyl C-5
13. Solvolytic desulfation of glycosaminoglycuronan sulfates with dimethyl sulphoxide containing water or methanol
14. Reactivity toward chemical sulfafion of hydroxyl groups of heparin
15. Novel regio- and stereoselective modification of heparin in alkaline solution: Nuclear magnetic resonance spectroscopic evidence
16. Sulfation of some chemically modified heparins. Formation of a 3-sulfate analog of heparin
17. 13C NMR spectral assignments of the major sequences of twelve systematically modified heparin derivatives
18. Changes in the chemical structure and biological property of NO sulfate-transferred, N-resulfated heparin
19. Characterization of heparins by high performance size exclusion liquid chromatography
20. "Supersulfated" heparin fragments, a new type of low-molecular weight heparin
21. A simple conductimetric method for determination of carboxyl and sulfate groups of heparin and other glycosaminoglycans
22. Evaluation of an amidolytic heparin assay method: Increased sensitivity by adding purified antithrombin III
23. Extension and structural variability of the antithrombin-binding sequence in heparin