Studies on trypsin-modified bovine and human lens acylpeptide hydrolase

Experimental Eye Research

Volumn 72, Issue 3, 2001, Pages 301-310

  Senthilkumar, R ^a   Neelakanta Reddy, P ^a   Krishna Sharma, K ^a,b  

^a UNIVERSITY OF MISSOURI   (United States)

^b University of Missouri   (United States)

Author keywords

Cleavage; Crystallins; Enzyme; Inhibitors; Peptide; Proteins

Indexed keywords

4 CHLOROMERCURIBENZOIC ACID; ACYLAMINO ACID RELEASING ENZYME; BETA CRYSTALLIN; DYFLOS; EBELACTONE A; GAMMA CRYSTALLIN; PROTEINASE; TOSYLPHENYLALANYL CHLOROMETHYL KETONE; TRYPSIN;

ADOLESCENT; ADULT; AGED; AMINO ACID SEQUENCE; ANIMAL TISSUE; ARTICLE; CATTLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME SUBSTRATE; HUMAN; HUMAN TISSUE; HYDROLYSIS; LENS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN MODIFICATION; PROTEIN PROCESSING;

EID: 0034769591     PISSN: 00144835     EISSN: None     Source Type: Journal    
DOI: 10.1006/exer.2000.0955     Document Type: Article

References (39)

1. The interaction of α2-macroglobulin with proteinases. Characterestics and specificity of the reaction and a hypothesis concerning its molecular mechanism
2. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels
3. Characterization of trypsin-modified bovine lens acylpeptide hydrolase
4. Cataract and the acceleration of calpain-induced beta-crystallin insolubilization occurring during normal maturation of rat lens
5. Role of proteolysis in lenses
6. Sequence analysis of lens beta-crystallins suggests involvement of calpain in cataract formation
7. Crystallization and preliminary X-ray studies of human erythrocyte acylpeptide hydrolase
8. Age changes in bovine lens endopeptidase activity
9. Purification and partial characterization of alpha-N acylpeptide hydrolase from bovine liver
10. Aging and cellular maturation cause changes in ubiquitin-eye lens protein conjugates
11. Acylpeptide hydrolase activity from erythrocytes
12. Cleavage of amino acid residue(s) from the N-terminal region of alpha A- and alpha B-crystallins in human crystalline lens during aging
13. Acyl-peptide hydrolase from rat liver. Characterization of enzyme reaction
14. Age-dependent changes in the heat-stable crystallin, beta Bp, of the human lens
15. The primary structure of porcine liver acylamino acid releasing enzyme deduced from cDNA sequences
16. Complete covalent structure of porcine liver acylamino acid-releasing enzyme and identification of its active site serine residue
17. Purification and crystallization of human carboxypeptidase A
18. Cleavage of the Lys196-Ser197 bond of prolyl oligopeptidase: Enhanced catalytic activity for one of the two active enzyme forms
19. The glycation and cross-linking of isolated lens crystallins by ascorbic acid
20. Purification and characterization of an N-acylaminoacyl-peptide hydrolase from rabbit muscle
21. A new family of serine-type peptidases related to prolyl oligopeptidase
22. Human insoluble lens protein. II. Isolation and characterization of a 9600 dalton polypeptide
23. Human acylpeptide hydrolase. Studies on its thiol groups and mechanism of action
24. Structural investigations on human erythrocyte acylpeptide hydrolase by mass spectrometric procedures
25. Acylpeptide hydrolase: Inhibitors and some active site residues of the human enzyme
26. Peptide hydrolysis in lens: Role of leucine aminopeptidase, aminopeptidase III, prolyloligopeptidase and acylpeptidehydrolase
27. Description of an acylpeptide hydrolase from lens
28. Bovine lens acylpeptide hydrolase. Purification and characterization of a tetrameric enzyme resistant to urea denaturation and proteolytic inactivation
29. Cleavage of beta crystallins during maturation of bovine lens
30. Measurement of protein using bicinchoninic acid
31. Age-related increase in concentration and aggregation of degraded polypeptides in human lenses
32. Isolation and characterization of a 25 K serine proteinase from bovine lens cortex
33. Characterization of a sodium deoxycholate-activatable proteinase activity associated with βA3/A1-crystallin of human lenses
34. Cleavage from the N-terminal region of βBp crystallin during aging of the human lens
35. Cataract: Relationship between nutrition and oxidation
36. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
37. Purification and properties of acylamino acid-releasing enzyme from rat liver
38. Ebelactone, an inhibitor of esterase, produced by actinomycetes
39. Calpain and alpha-crystallin in the lens of cac mouse