Influence of a sulfhydryl cross-link across the allosteric-site interface of E. coli phosphofructokinase

Protein Science

Volumn 10, Issue 11, 2001, Pages 2186-2194

  Johnson, Jason L ^a   Lasagna, Mauricio D ^b   Reinhart, Gregory D ^b  

^a SOUTHWESTERN OKLAHOMA STATE UNIVERSITY   (United States)

^b TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Allosteric regulation; Disulfide cross link; Hybrid formation; Phosphofructokinase; Pressure dissociation; Subunit interactions; Urea denaturation

Indexed keywords

6 PHOSPHOFRUCTOKINASE; CYSTEINE; DITHIOTHREITOL; MONOMER; N ETHYLMALEIMIDE; THIOL DERIVATIVE;

ALLOSTERISM; ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; CROSS LINKING; DISULFIDE BOND; ENZYME BINDING; ENZYME SUBSTRATE; ESCHERICHIA COLI; HYBRID; NONHUMAN; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN DENATURATION; PROTEIN INTERACTION; PROTEIN STABILITY;

ALLOSTERIC REGULATION; ALLOSTERIC SITE; BINDING SITES; DISULFIDES; DITHIOTHREITOL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; ENZYME STABILITY; ESCHERICHIA COLI; PHOSPHOFRUCTOKINASE-1; PRESSURE; PROTEIN FOLDING; SULFHYDRYL REAGENTS; UREA;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0034769502     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.02401     Document Type: Article

References (28)

1. Disulfide bonds and the stability of globular proteins
2. Kinetics of the allosteric interactions of phosphofructokinase from Escherichia coli
3. The kinetics of enzyme-catalyzed reactions with two or more substrates of products. I. Nomenclature and rate equations
4. Molecular cloning of the gene for phosphofructokinase-2 of Escherichia coli and the nature of a mutation, pfkB1, causing a high level of the enzyme
5. Reversible high hydrostatic pressure inactivation of phosphofructokinase from Escherichia coli
6. Ordered disruption of subunit interfaces during the stepwise reversible dissociation of Eschericia coli phosphofructokinase with KSCN
7. Theory of elastic mechanisms in fibrous proteins
8. Nucleotide sequence and high-level expression of the major Escherichia coli phosphofructokinase
9. Protein structure and dynamics at high pressure
10. MgATP and fructose 6-phosphate interactions with phosphofructokinase from Escherichia coli
11. Influence of MgADP on phosphofructokinase from Escherichia coli elucidation of coupling interactions with both substrates
12. High pressure effects on E. coli phosphofructokinase
13. Failure of a two-state model to describe the influence of phospho(enol)pyruvate on phosphofructokinase from Escherichia coli
14. Two Escherichia coli fructose-6-phosphate kinases: Preparative purification, oligomeric structure, and immunological studies
15. Phosphofructokinases from Escherichia coli
16. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
17. Tetramer-dimer equilibrium of phosphofructokinase and formation of hybrid tetramers
18. Urea-induced inactivation, dissociation, and unfolding of the allosteric phosphofructokinase from Escherichia coli
19. The determination of thermodynamic allosteric parameters of an enzyme undergoing steady-state turnover
20. Influence of pH on the regulatory kinetics of rat liver phosphofructokinase: A thermodynamic linked-function analysis
21. Linked-function origins of cooperativity in a symmetrical dimer
22. Structural basis of the allosteric behavior of phosphofructokinase
23. Crystal structure of the complex of phosphofructokinase from Escherichia coli with its reaction products
24. Intermediates on the reassociation pathway of phosphofructokinase I from Escherichia coli
25. Ligand binding and internal equilibria in proteins
26. Energetics of ligand binding to proteins
27. Tetramer-dimer conversion of phosphofructokinase from Thermus thermophilus induced by its allosteric effectors
28. The mechansim of ATP inhibition of wild type and mutant phosphofructo-1-kinase from Escherichia coli