Import of colicins across the outer membrane of Escherichia coli involves multiple protein interactions in the periplasm

Molecular Microbiology

Volumn 42, Issue 2, 2001, Pages 331-344

  Journet, Laure ^a,b   Bouveret, Emmanuelle ^a   Rigal, Alain ^a   Lloubes, Roland ^a   Lazdunski, Claude ^a   Bénédetti, Hélène ^a,c  

^a CNRS   (France)

^b UNIVERSITY OF BASEL   (Switzerland)

^c CENTRE DE BIOPHYSIQUE MOLÉCULAIRE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

COLICIN A; ENVELOPE PROTEIN; ENVELOPE PROTEIN TOLA; ENVELOPE PROTEIN TOLB; ENVELOPE PROTEIN TOLR; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL MEMBRANE; BACTERIAL OUTER MEMBRANE; CONTROLLED STUDY; CYTOPLASM; ESCHERICHIA COLI; IN VITRO STUDY; IN VIVO STUDY; MEMBRANE TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN TRANSPORT;

BACTERIAL PROTEINS; CELL MEMBRANE; COLICINS; CROSS-LINKING REAGENTS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FORMALDEHYDE; MEMBRANE PROTEINS; PERIPLASM; PERIPLASMIC PROTEINS; PLASMIDS; PRECIPITIN TESTS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; GOSSWEILERODENDRON BALSAMIFERUM; NEGIBACTERIA;

EID: 0034767924     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2001.02592.x     Document Type: Article

References (60)

1. Crystallization and preliminary crystallographic study of a component of the Escherichia coli tol system: TolB
2. Displacement of OmpF loop 3 is not required for the membrane translocation of colicins N and A in vivo
3. Functional domains of colicin A
4. Individual domains of colicins confer specificity in colicin uptake, in pore-properties and in immunity requirement
5. Protein import into Escherichia coli: Colicins A and E1 interact with a component of their translocation system
6. Colicin A unfolds during its translocation in Escherichia coli cells and spans the whole cell envelope when its pore has formed
7. Escherichia coli tol-pal mutants form outer membrane vesicles
8. In vitro characterization of peptido-glycan-associated lipoprotein (PAL) - Peptidoglycan and PAL-TolB interactions
9. Peptidoglycan-associated lipoprotein-TolB interaction. A possible key to explaining the formation of contact sites between the inner and outer membranes of Escherichia coli
10. The N-terminal domain of colicin E3 interacts with TolB which is involved in the colicin translocation step
11. Distinct regions of the colicin A translocation domain are involved in the interaction with TolA and TolB proteins upon import into Escherichia coli
12. Evolutionary relationship of uptake systems for biopolymers in Escherichia coli: Cross-complementation between the TonB-ExbB-ExbD and the TolA-TolQ-TolR proteins
13. Site-directed disulfide bonding reveals an interaction site between energy-coupling protein TonB and BtuB, the outer membrane cobalamin transporter
14. A new family of sugar-inducible expression vectors for Escherichia coli
15. The structure of TolB, an essential component of the tol-dependent translocation system, and its protein-protein interaction with the translocation domain of colicin E9
16. Proton motive force drives the interaction of the inner membrane TolA and outer membrane pal proteins in Escherichia coli
17. A molecular, genetic and immunological approach to the functioning of colicin A, a pore-forming protein
18. Purification and molecular properties of a new colicin
19. TolB protein of Escherichia coli K-12 interacts with the outer membrane peptidoglycan-associated proteins Pal, Lpp and OmpA
20. Genetics of resistance to colicins in Escherichia coli K-12: Cross- resistance among colicins of group A
21. Genetics of resistance to colicins in Escherichia coli K-12: Cross- resistance among colicins of group B
22. Protein complex within Escherichia coli inner membrane. TolA N-terminal domain interacts with TolQ and TolR proteins
23. TolA central domain interacts with Escherichia coli porins
24. Binding of colicins A and E1 to purified TolA domains
25. Quantification of group A colicin import sites
26. Import of biopolymers into Escherichia coli: Nucleotide sequences of the exbB and exbD genes are homologous to those of the tolQ and tolR genes, respectively
27. Specific regions of Escherichia coli OmpF protein involved in antigenic and colicin receptor sites and in stable trimerization
28. Construction, expression and release of hybrid colicins
29. Surface expression of O-specific lipopolysaccharide in Escherichia coli requires the function of the TolA protein
30. An alpha-helical hydrophobic hairpin as a specific determinant in protein-protein interaction occurring in Escherichia coli colicin A and B immunity systems
31. Mutational analysis of the Escherichia coli K-12 TolA N-terminal region and characterization of its TolQ-interacting domain by genetic suppression
32. Aminopeptidase N- and human blood group A-antigenicity along the digestive tract and associated glands in the rabbit
33. Colicin A and the Tol proteins involved in its translocation are preferentially located in the contact sites between the inner and outer membranes of Escherichia coli cells
34. Site-directed mutagenesis by overlap extinction using the polymerase chain reaction
35. Maturation and localization of the TolB protein required for colicin import
36. Role of TolR N-terminal, central, and C-terminal domains in dimerization and interaction with TolA and tolQ
37. Conformation of colicin A: Apparent difference between cytoplasmic and extracellular polypeptide chain
38. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
39. Colicin import into Escherichia coli cells
40. The excC gene of Escherichia coli K-12 required for cell envelope integrity encodes the peptidoglycan-associated lipoprotein (PAL)
41. Genetic and biochemical characterization of periplasmic-leaky mutants of Escherichia coli K-12
42. Transmembrane alpha-helix interactions are required for the functional assembly of the Escherichia coli Tol complex
43. TolA: A membrane protein involved in colicin uptake contains an extended helical region
44. BiP acts as a molecular ratchet during posttranslational transport of prepro-alpha factor across the ER membrane
45. A short course in bacterial genetics
46. TonB-dependent iron acquisition: Mechanisms of siderophore-mediated active transport
47. Membrane topology of the Escherichia coli TolR protein required for cell envelope integrity
48. A new method for strand discrimination in sequence-directed mutagenesis
49. Strong function-related homology between the pore-forming colicins K and 5
50. Structural and functional properties of colicin B
51. Discovery of critical Tol A-binding residues in the bactericidal toxin colicin N: A biophysical approach
52. The TolB protein interacts with the porins of Escherichia coli
53. DNA sequencing with chain-terminating inhibitors
54. Nucleotide sequence of the colicin B activity gene cba: Consensus pentapeptide among TonB-dependent colicins and receptors
55. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications
56. Crystal structure of a colicin N fragment suggests a model for toxicity
57. Characterization of the tol-pal region of Escherichia coli K-12: Translational control of tolR expression by TolQ and identification of a new open reading frame downstream of pal encoding a periplasmic protein
58. Crystal structure of colicin Ia