A monoclonal antibody specific for Pseudomonas aeruginosa amidase

Hybridoma

Volumn 20, Issue 4, 2001, Pages 273-279

  Novo, C ^a   Karmali, A ^a   Clemente, A ^a   Brown, P R ^a  

^a INETI   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

AMIDASE; BACTERIAL ENZYME; IMMUNOGLOBULIN M ANTIBODY; IMMUNOGLOBULIN SUBCLASS; MONOCLONAL ANTIBODY;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANION EXCHANGE CHROMATOGRAPHY; ANTIGEN BINDING; ARTICLE; CELL FUSION; CELL LINE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME DENATURATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; FEMALE; HYBRIDOMA; IMMUNIZATION; MOLECULAR RECOGNITION; MOUSE; MYELOMA CELL; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; WESTERN BLOTTING;

AMIDOHYDROLASES; ANIMALS; ANTIBODIES, MONOCLONAL; ANTIBODY SPECIFICITY; BLOTTING, WESTERN; CHROMATOGRAPHY, ION EXCHANGE; FEMALE; IMMUNOGLOBULIN M; MICE; MICE, INBRED BALB C; PSEUDOMONAS AERUGINOSA;

ANIMALIA; BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0034766855     PISSN: 0272457X     EISSN: None     Source Type: Journal    
DOI: 10.1089/027245701753179866     Document Type: Article

References (19)

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4. The nucleotide sequence of the amiE gene of Pseudomonas aeruginosa
5. Cloning and primary structure of the wide-spectrum amidase from Brevibacterium sp. R312: High homology to the amiE product from Pseudomonas aeruginosa
6. The complete genome sequence of the gastric pathogen Helicobacter pylori
7. Cloning of an Aliphatic Amidase from Bacillus stearothermophilus BR388 in Escherichia coli and marked enhancement of amidase expression using directed evolution
8. Directed evolution of amidase in Methylophiles methylotrophus, purification and properties of amidases from wild-type and mutant strains
9. Evolution in action
10. Amino acid substitution in an amidase produced by an acetanilide-utilizing mutant of Pseudomonas aeruginosa
11. Molecular basis of altered enzyme specificities in a family of mutant amidases
12. Arg-188 and Trp-144 are implicated in the binding of urea and acetamide to the active site of the amidase from Pseudomonas aeruginosa
13. Substitution of Glu-59 by Val in amidase from Pseudomonas aeruginosa results in a catalytically inactive enzyme
14. A monoclonal antibody for large-scale purification of human leukocyte interferon
15. Determination of submicro quantities of ammonia
16. Techniques for the production and characterization of monoclonal hybridoma antibodies