Aggregation and chemical reaction in hen lysozyme caused by heating at pH 6 are depressed by osmolytes, sucrose and trehalose

Journal of Biochemistry

Volumn 130, Issue 4, 2001, Pages 491-496

  Ueda, T ^a   Nagata, M ^a   Imoto, T ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Aggregation; Deamidation; Hen lysozyme; Racemization; Sucrose; Trehalose

Indexed keywords

LYSOZYME; SUCROSE; TREHALOSE;

ARTICLE; ENZYME INACTIVATION; ENZYME STABILITY; NONHUMAN; PROTEIN AGGREGATION; PROTEIN CONFORMATION; PROTEIN TERTIARY STRUCTURE; THERMOSTABILITY;

EID: 0034764550     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003011     Document Type: Article

References (24)

1. Living with water stress: Evolution of osmolyte systems
2. Increased thermal stability of proteins in the presence of naturally occurring osmolytes
3. 2 system
4. Why preferential hydration does not always stabilize the native structure of globular proteins
5. The stabilization of proteins by sucrose
6. The control of protein stability and association by weak interactions with water: How do solvents affect these processes?
7. Why do some organisms use a urea-methylamine mixture as osmolyte? Thermodynamic compensation of urea and trimethylamine N-oxide interactions with protein
8. The mechanisms of irreversible enzyme inactivation at 100°C
9. The mechanism of irreversible inactivation of lysozyme at pH 4 and 100°C
10. Effects of additives on irreversible inactivation of lysozyme at neutral pH and 100°C
11. Stabilization of lysozyme against irreversible inactivation by suppression of chemical reactions
12. An S-alkylating reagent with positive charges as an efficient solubilizer of denatured disulfide-containing proteins
13. Preparation and properties of a lysozyme derivative in which two domains are cross-linked intramolecularly between Trp62 and Asp101
14. Purification and characterization of active and latent forms of recombinant plasminogen activator inhibitor 1 produced in Escherichia coli
15. Kinetics and thermodynamics of phalloidin binding to actin filaments from three divergent species
16. 13C-enriched methionine resonance
17. Unfolding of lysozyme by breaking its disulphide bridges results in exposure of hydrophobic sites
18. Vertebrate lysozyme
19. Foldingand aggregation of TEM beta-lactamase: Analogies with the formation of inclusion bodies in Escherichia coli
20. Kinetic study of the competition between renaturation and aggregation during the refoldingof denatured-reduced egg white lysozyme
21. Manipulating the amyloid-beta aggregation pathway with chemical chaperones
22. Forcing thermodynamically unfolded proteins to fold
23. Mechanism of protein stabilization by glycerol: Preferential hydration in glycerol-water mixtures
24. Molecular chaperones