Insertions in the gG gene of pseudorabies virus reduce expression of the upstream Us3 protein and inhibit cell-to-cell spread of virus infection

Journal of Virology

Volumn 75, Issue 22, 2001, Pages 10856-10869

  Demmin, G L ^a   Clase, A C ^a   Randall, J A ^a   Enquist, L W ^a   Banfield, B W ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN SERINE THREONINE KINASE; THROMBOSPONDIN; VIRUS PROTEIN;

ANIMAL TISSUE; ARTICLE; CELL SPREADING; EMBRYO; GENE DELETION; GENE EXPRESSION; GENE INSERTION; GENE LOCUS; HERPES VIRUS; NONHUMAN; NONSENSE MUTATION; PRIORITY JOURNAL; PSEUDORABIES HERPETOVIRUS; VIRUS INFECTION; VIRUS VIRULENCE;

ANIMALS; CHICK EMBRYO; CODON, NONSENSE; GENES, VIRAL; HERPESVIRUS 1, SUID; PROTEIN-SERINE-THREONINE KINASES; PSEUDORABIES VACCINES; RNA, MESSENGER; VIRAL ENVELOPE PROTEINS; VIRAL PROTEINS; VIRULENCE; VIRUS REPLICATION;

EID: 0034762655     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.22.10856-10869.2001     Document Type: Article

References (88)

1. Deletion of glycoprotein gE reduces the propagation of pseudorabies virus in the nervous system of mice after intranasal inoculation
2. An analysis of the in vitro and in vivo phenotypes of mutants of herpes simplex virus type 1 lacking glycoproteins gG, gE, gI or the putative gJ
3. A chicken embryo eye model for the analysis of alphaherpesvirus neuronal spread and virulence
4. Trypsin and heat resistance of some strains of the virus group
5. The processing of pseudorabies virus glycoprotein gX in infected cells and in an uninfected cell line
6. Synthesis of proteins in cells infected with herpesvirus. V. Viral glycoproteins
7. Role of pseudorabies virus us9, a type II membrane protein, in infection of tissue culture cells and the rat nervous system
8. Pseudorabies virus envelope glycoprotein gl influences both neurotropism and virulence during infection of the rat visual system
9. Identification of a target protein of US3 protein kinase of herpes simplex virus type 2
10. The US3 protein kinase of herpes simplex virus type 2 is associated with phosphorylation of the UL12 alkaline nuclease in vitro
11. Analysis of the contributions of herpes simplex virus type 1 membrane proteins to the induction of cell-cell fusion
12. Pseudorabies virus glycoprotein K requires the UL20 gene product for processing
13. Intracellular processing of pseudorabies virus glycoprotein M (gM): gM of strain Bartha lacks N-glycosylation
14. Herpes simplex virus glycoproteins E and I facilitate cell-to-cell spread in vivo and across junctions of cultured cells
15. Glycoproteins E and I facilitate neuron-to-neuron spread of herpes simplex virus
16. Infection and spread of alphaherpesviruses in the nervous system
17. Genetic analysis of the role of herpes simplex virus type 1 glycoprotein K in infectious virus production and egress
18. Identification of the herpes simplex virus protein kinase as the product of viral gene US3
19. Herpes simplex virus 1 induces and blocks apoptosis at multiple steps during infection and protects cells from exogenous inducers in a cell-type-dependent manner
20. The UL45 gene product is required for herpes simplex virus type 1 glycoprotein B-induced fusion
21. Antiapoptotic activity of herpes simplex virus type 2: The role of US3 protein kinase gene
22. Herpes simplex virus glycoprotein K is known to influence fusion of infected cells, yet is not on the cell surface
23. Herpes simplex virus inhibits apoptosis through the action of two genes, Us5 and Us3
24. Herpes simplex virus gE/gI sorts nascent virions to epithelial cell junctions, promoting virus spread
25. Circuit-specific coinfection of neurons in the rat central nervous system with two pseudorabies virus recombinants
26. Inactivation of glycoprotein gE and thymidine kinase or the US3-encoded protein kinase synergistically decreases in vivo replication of pseudorabies virus and the induction of protective immunity
27. Contributions of single genes within the unique short region of Aujeszky's disease virus (suid herpes virus 1) to virulence, pathogenesis and immunogenicity
28. Pseudorabies virus glycoprotein gK is a virion structural component involved in virus release but is not required for entry
29. Primary envelopment of pseudorabies virus at the nuclear membrane requires the UL34 gene product
30. Mutations affecting the UL21 gene contribute to avirulence of pseudorabies virus vaccine strain Bartha
31. Pseudorabies virus glycoprotein M inhibits membrane fusion
32. Pseudorabies virus recombinants expressing functional virulence determinants gE and gI from bovine herpes-virus 1.1
33. The gE and gI homologs from two alphaherpesviruses have conserved and divergent neuroinvasive properties
34. Dissemination of wild-type gC-, gE-, and gI-deleted mutants of Aujeszk-y's disease virus in the maxillary nerve and trigeminal ganglion of pigs after intranasal inoculation
35. Invasion and spread of single glycoprotein deleted mutants of Aujeszky's disease virus (ADV) in the trigeminal nervous pathway of pigs after intranasal inoculation
36. Role of envelope glycoproteins gI, gp63 and gIII in the invasion and spread of Aujeszky's disease virus in the olfactory nervous pathway of the pig
37. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
38. The herpes simplex virus 1 protein kinase US3 is required for protection from apoptosis induced by the virus
39. The complete DNA sequence and the genetic organization of the short unique region (US) of the bovine herpesvirus type 1 (ST strain)
40. Deletions in the genomes of pseudorabies virus vaccine strains and existence of four isomers of the genomes
41. Multiple defects in the genome of pseudorabies virus can affect virulence without detectably affecting replication in cell culture
42. Genetic basis of the neurovirulence of pseudorabies virus
43. Genome location and identification of functions defective in the Bartha vaccine strain of pseudorabies virus
44. Clustering of genes dispensable for growth in culture in the S component of the HSV-1 genome
45. Alphaherpesviruses possess a gene homologous to the protein kinase gene family of eukaryotes and retroviruses
46. Cytoplasmic domain of herpes simplex virus gE causes accumulation in the trans-Golgi network, a site of virus envelopment and sorting of virions to cell junctions
47. Pseudorabies virus avirulent strains fail to express a major glycoprotein
48. A glycoprotein gX-β-galactosidase fusion gene as insertional marker for rapid identification of pseudorabies virus mutants
49. Role of a structural glycoprotein of pseudorabies in virus virulence
50. Characterization of cell-cell fusion mediated by herpes simplex virus 2 glycoproteins gB, gD, gH and gL in transfected cells
51. Glycoprotein D-negative pseudorabies virus can spread transneuronally via direct neuron- to- neuron transmission in its natural host, the pig, but not after additional inactivation of gE or gI
52. Glycoprotein gE-negative pseudorabies virus has a reduced capability to infect second- and third-order neurons of the olfactory and trigeminal routes in the porcine central nervous system
53. Pseudorabies virus infections in pigs. Role of viral proteins in virulence, pathogenesis and transmission
54. Bovine herpesvirus 1 glycoprotein G is required for prevention of apoptosis and efficient viral growth in rabbit kidney cells
55. Bovine herpes-virus 1 glycoprotein G is required for viral growth by cell-to-cell infection
56. Construction of a US3 lacZ insertion mutant of herpes simplex virus type 2 and characterization of its phenotype in vitro and in vivo
57. Envelope glycoprotein gp50 of pseudorabies virus is essential for virus entry but is not required for viral spread in mice
58. Morphogenesis of three pseudorabies virus strains in porcine nasal mucosa
59. The herpes simplex virus 1 protein kinase encoded by the US3 gene mediates posttranslational modification of the phosphoprotein encoded by the UL34 gene
60. Glycoprotein K of herpes simplex virus: A transmembrane protein encoded by the UL53 gene which regulates membrane fusion
61. Mutations in herpes simplex virus glycoprotein D distinguish entry of free virus from cell-cell spread
62. Mapping and sequence of the gene for the pseudorabies virus glycoprotein which accumulates in the medium of infected cells
63. A simple method of estimating fifty percent endpoints
64. Detailed analysis of the mRNAs mapping in the short unique region of herpes simplex virus type 1
65. The gene encoding the gIII envelope protein of pseudorabies virus vaccine strain Bartha contains a mutation affecting protein localization
66. Polykaryocytosis
67. Herpes simplex virus type 1 U(L)34 gene product is required for viral envelopment
68. Requirement of interaction of nectin-1α/HveC with afadin for efficient cell-cell spread of herpes simplex virus type 1
69. Herpes simplex virus 1 alkaline nuclease is required for efficient egress of capsids from the nucleus
70. Pseudorabies virus expressing enhanced green fluorescent protein: A tool for in vitro electrophysiological analysis of transsynaptically labeled neurons in identified central nervous system circuits
71. A self-recombining bacterial artificial chromosome and its application for analysis of herpesvirus pathogenesis
72. Entry of alphaherpesvirus into cells
73. Membrane fusion induced by herpes simplex virus
74. Dendritic morphology of cardiac related medullary neurons defined by circuit-specific infection by a recombinant pseudorabies virus expressing β-galactosidase
75. Nectin/ PRR: An immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with Afadin, a PDZ domain-containing protein
76. HveA (herpesvirus entry mediator A), a coreceptor for herpes simplex virus entry, also participates in virus-induced cell fusion
77. Replication and virulence of pseudorabies virus mutants lacking glycoprotein gX
78. Characterization of pseudorabies virus mutants expressing carboxy-terminal truncations of gE: Evidence for envelope incorporation, virulence, and neurotropism domains
79. A herpes simplex virus 1 recombinant lacking the glycoprotein G coding sequences is defective in entry through apical surfaces of polarized epithelial cells in culture and in vivo
80. Glycoproteins gB, gD, and gHgL of herpes simplex virus type 1 are necessary and sufficient to mediate membrane fusion in a Cos cell transfection system
81. Identification of two genes in the unique short region of pseudorabies virus; comparison with herpes simplex virus and varicella-zoster virus
82. The US3-encoded protein kinase from pseudorabies virus affects egress of virions from the nucleus
83. Rapid identification of nonessential genes of herpes simplex virus type 1 by Tn5 mutagenesis
84. Specific pseudorabies virus infection of the rat visual system requires both gl and gp63 glycoproteins
85. The extra-cellular domain of herpes simplex virus gE is sufficient for accumulation at cell junctions but not for cell-to-cell spread
86. The protein kinase encoded in the short unique region of pseudorabies virus: Description of the gene and identification of its product in virions and in infected cells
87. Glycoprotein gI of pseudorabies virus promotes cell fusion and virus spread via direct cell-to-cell transmission