Methylation of CpG dinucleotides alters binding and silences testis-specific transcription directed by the mouse lactate dehydrogenase C promoter

Biology of Reproduction

Volumn 65, Issue 5, 2001, Pages 1522-1527

  Kroft, T L ^a   Jethanandani, P ^a   McLean, D J ^a   Goldberg, E ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Cyclic adenosine monophosphate; Gene regulation; Spermatogenesis; Testis

Indexed keywords

CYCLIC AMP; DINUCLEOTIDE; GENE PRODUCT; LACTATE DEHYDROGENASE ISOENZYME; LACTATE DEHYDROGENASE ISOENZYME 4; PROTEIN MLDHC; TRANSCRIPTION FACTOR; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING SITE; CONTROLLED STUDY; CPG ISLAND; DNA BINDING; ELECTROPHORETIC MOBILITY; GENE ACTIVATION; GENE CONTROL; GENETIC ANALYSIS; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; GERM LAYER; MALE; METHYLATION; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; SILENCER GENE; SPERMATOGENESIS; TANDEM REPEAT; TESTIS; TRANSCRIPTION REGULATION; TRANSCRIPTION TERMINATION; TRANSGENIC MOUSE;

ANIMALIA; MUS MUSCULUS;

EID: 0034762421     PISSN: 00063363     EISSN: None     Source Type: Journal    
DOI: 10.1095/biolreprod65.5.1522     Document Type: Article

References (18)

1. Methylation patterns of testis-specific genes
2. The CpG-rich promoter of human LDH-C is differentially methylated in expressing and nonexpressing tissues
3. DNA methylation is the primary silencing mechanism for a set of germ line- and tumor-specific genes with a CpG-rich promoter
4. Methylation-dependent silencing of the testis-specific Pdha-2 basal promoter occurs through selective targeting of an activating transcription factor/cAMP-responsive element-binding site
5. Regulation of Pdha-2 expression is mediated by proximal promoter sequences and CpG methylation
6. Differential methylation in steroid 5 alpha-reductase isozyme genes in epididymis, testis, and liver of the adult rat
7. Regulators for the rat clusterin gene: DNA methylation and cis-acting regulatory elements
8. A genespecific promoter in transgenic mice directs testis-specific demethylation prior to transcriptional activation in vivo
9. A 60-bp core promoter sequence of murine lactate dehydrogenase c is sufficient to direct testis-specific transcription in vitro
10. Transgenic mice demonstrate a testis-specific promoter for lactate dehydrogenase, LDHC
11. Use of a HpaII-polymerase chain reaction assay to study DNA methylation in the Pgk-1 CpG island of mouse embryos at the time of X-chromosome inactivation
12. High sensitivity mapping of methylated cytosines
13. Suppression of metallothionein gene expression in a rat hepatoma because of promoter-specific DNA methylation
14. Site-specific methylation of the promoter alters deoxyribonucleic acid-protein interactions and prevents follicle-stimulating hormone receptor gene transcription
15. Transcription of the testis-specific mouse protamine 2 gene in a homologous in vitro transcription system
16. Tissue-specific in vitro transcription from the mouse albumin promoter
17. A dual-function palindromic sequence regulates testis-specific transcription of the mouse lactate dehydrogenase c gene in vitro