Effects of flavin-binding motif amino acid mutations in the NADH-cytochrome b5 reductase catalytic domain on protein stability and catalysis

Journal of Biochemistry

Volumn 130, Issue 4, 2001, Pages 481-490

  Kimura, Shigenobu ^a   Nishida, Hirokazu ^a,b   Iyanagi, Takashi ^a  

^a UNIVERSITY OF HYOGO   (Japan)

^b HITACHI LTD   (Japan)

Author keywords

Electron transfer; Flavin binding motif; NADH cytochrome b5 reductase, protein stability; Site directed mutagenesis

Indexed keywords

CYTOCHROME B5 REDUCTASE;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; ELECTRON TRANSPORT; NONHUMAN; OXIDATIVE PHOSPHORYLATION; PROTEIN STABILITY; SITE DIRECTED MUTAGENESIS; YEAST;

EID: 0034759905     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a003010     Document Type: Article

References (40)

1. 5 reductase containing both the catalytic site and an additional hydrophobic membrane-binding segment
2. 5 in fatty acid desaturation by rat liver microsomes
3. 5 reductase
4. 5 reductase from human erythrocytes
5. 5 reductase at 2.5 Å resolution
6. 5 reductase from pig liver microsomes
7. 5 reductase from pig liver at 2.4 Å resolution
8. 5 reductase and the other flavin-dependent reductases
9. 5 reductase and the other flavin-dependent reductases: Pyridine nucleotide-binding and interaction with an electron-transfer partner
10. + reductase: Prototype for a structurally novel flavoenzyme family
11. Phthalate dioxygenase reductase: A modular structure for electron transfer from pyridine nucleotides to [2Fe.2S]
12. Crystal structure of the FAD-containing fragment of corn nitrate reductase at 2.5 Å resolution: Relationship to other flavoprotein reductases
13. Structural studies on corn nitrate reductase: Refined structure of the cytochrome b reductase fragment at 2.5 Å, its ADP complex and an active-site mutant and modeling of the cytochrome b domain
14. The three-dimensional structure of flavodoxin reductase from Escherichia coli at 1.7 Å resolution
15. Structural prototypes for an extended family of flavoprotein reductases: Comparison of phthalate dioxygenase reductase with ferredoxin reductase and ferredoxin
16. 5 reductase
17. 5 reductase purified from rabbit erythrocytes
18. 5 reductase in electron transfer reaction
19. 5 reductase
20. Three-dimensional structure of NADPH-cytochrome P450 reductase: Prototype for FMN- and FAD-containing enzymes
21. 5 reductase solubilized domain
22. 5 and NADPH-cytochrome c reductase from rat liver microsomes
23. Recombinant PCR
24. Extraction, purification, and separation of tissue flavins for spectrophotometric determination
25. + reductase from spinach leaves
26. Circular dichroism studies of the flavin chromophore and of the relation between redox properties and flavin environment in oxidases and dehydrogenases
27. Molecular luminescence studies of flavins. I. The excited states of flavins
28. The intraflavin hydrogen bond in human electron transfer flavoprotein modulates redox potentials and may participate in electron transfer
29. Principles and patterns of protein conformation
30. + reductase revealed by protein engineering and crystallographic studies
31. 5 reductase
32. 5 reductase
33. 5 by site-directed mutagenesis of an expression vector for the flavoprotein
34. 5 studied by site-directed mutagenesis
35. 5 reductase causes hereditary methemoglobinemia, generalized type
36. Site-directed mutagenesis of tyrosine-98 in the flavodoxin from Desulfovibrio vulgaris (Hildenborough): Regulation of oxidation-reduction properties of the bound FMN cofactor by aromatic, solvent, and electrostatic interactions
37. Differential stabilization of the three FMN redox forms by tyrosine 94 and tryptophan 57 in flavodoxin from Anabaena and its influence on the redox potentials
38. Crystal structure of NAD(P)H:flavin oxidoreductase from Escherichia coli
39. Characterization of the flavoprotein moieties of NADPH-sulfite reductase from Salmonella typhimurium and Escherichia coli. Physicochemical and catalytic properties, amino acid sequence deduced from DNA sequence of cysJ, and comparison with NADPH-cytochrome P-450 reductase
40. cDNA cloning of human liver monoamine oxidase A and B: Molecular basis of differences in enzymatic properties