Antisense expression of the 20-hydroxyecdysone receptor (EcR) in transfected mosquito cells uncovers a new EcR isoform that varies at the C-terminal end

In Vitro Cellular and Developmental Biology - Animal

Volumn 37, Issue 8, 2001, Pages 522-529

  Jayachandran, G ^a   Fallon, A M ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Aedes albopictus; Antisense ribonucleic acid; C7 10 cell line; Ecdysone receptor; Transfection

Indexed keywords

COMPLEMENTARY RNA; ECDYSTERONE; ECDYSTERONE RECEPTOR; STEROID HORMONE; UNCLASSIFIED DRUG;

AEDES ALBOPICTUS; AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ANALYSIS; GENETIC TRANSFECTION; NONHUMAN; POLYMERASE CHAIN REACTION;

AEDES; AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CELLS, CULTURED; CLONING, MOLECULAR; CULICIDAE; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PROTEIN ISOFORMS; RECEPTORS, STEROID; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, ANTISENSE; TRANSFECTION;

AEDES ALBOPICTUS; ANIMALIA; INSECTA;

EID: 0034758090     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1290/1071-2690(2001)037<0522:AEOTHR>2.0.CO;2     Document Type: Article

References (30)

1. Cloning of human mineralocorticoid receptor complementary DNA: Structural and functional kinship with the glucocorticoid receptor
2. Mosquito ecdysteroid receptor: Analysis of the cDNA and expression during vitellogenesis
3. Transient expression of genes introduced into cultured cells of Drosophila
4. Plaque formation and isolation of pure lines of poliomyletis virus
5. The biochemistry and genetics of mosquito cells in culture
6. Cell cycle parameters in Aedes albopictus mosquito cells
7. Structure and chromosomal localization of the functional intronless human JUN protooncogene
8. Insect nuclear receptors: A developmental and comparative perspective
9. Cloning of a Chironomus tentans cDNA encoding a protein (cEcRH) homologous to the Drosophila melanogaster ecdysteroid receptor (dEcR)
10. Evidence for expression of EcR and USP components of the 20-hydroxyecdysone receptor by a mosquito cell line
11. Developmental profiles and ecdysteroid regulation of the mRNAs for two ecdysone receptor isoforms in the epidermis and wings of the tobacco hornworm, Manduca sexta
12. Tissue-specific and stage-specific expression of two silkworm ecdysone receptor isoforms - Ecdysteroid-dependent transcription in cultured anterior silk glands
13. Extensive regions of homology in front of the two hsp 70 heat shock variant genes in Drosophila melanogaster
14. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
15. Cultured Aedes albopictus mosquito cells synthesize hormone-inducible proteins
16. Inhibition of heat shock protein synthesis by heat-inducible antisense RNA
17. Comparison of the sevenless genes of Drosophila virilis and Drosophila melanogaster
18. Two distinct subpopulations of ecdysone receptor complex in the female mosquito during vitellogenesis
19. Cloning of two putative ecdysteroid receptor isoforms from Tenebrio molitor and their developmental expression in the epidermis during metamorphosis
20. Studies on two ecdysone receptor isoforms of the spruce budworm, Choristoneura furniferana
21. Analysis of a vitellogenin gene of the mosquito, Aedes aegypti and comparisons to vitellogenins from other organisms
22. Production of phenocopies by Krüppel antisense RNA injection into Drosophila embryos
23. The mosquito dihydrofolate reductase gene functions as a dominant selectable marker in transfected cells
24. Expression of an antisense dihydrofolate reductase transcript in transfected mosquito cells: Effects on growth and plating efficiency
25. The silkmoth homolog of the Drosophila ecdysone receptor (B1 isoform): Cloning and analysis of expression during follicular cell differentiation
26. Drosophila tissues with different metamorphic responses to ecdysone express different ecdysone receptor isoforms
27. Homeotic genes of the Bithorax complex repress limb development in the abdomen of the Drosophila embryo through the target gene distal-less
28. DNA binding and transactivation characteristics of the mosquito ecdysone receptor-ultraspiracle complex
29. The chromatin structure of specific genes: Evidence for higher order domains of defined DNA sequence
30. SREBP-1, a basic helix-loop-helix-helix-leucine zipper protein that controls transcription of the low density lipoprotein receptor gene