Two hydrophobic protein fractions of ovine pulmonary surfactant: Isolation, characterization, and biophysical activity

Protein Expression and Purification

Volumn 23, Issue 2, 2001, Pages 319-327

  Bünger, Harald ^a   Krüger, Ralph Peter ^b   Pietschmann, Sylvia ^a   Wüstneck, Nadeshda ^a   Kaufner, Lutz ^a   Tschiersch, Renate ^a   Pison, Ulrich ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b BAM FEDERAL INSTITUTE FOR MATERIALS RESEARCH AND TESTING   (Germany)

Author keywords

Captive bubble; MALDI TOF MS; Preparative HPLC; SP B; SP C; Surface pressure; Surfactant proteins

Indexed keywords

ANALYTIC METHOD; CHROMATOGRAPHY; DROP SHAPE ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBICITY; LIQUID CULTURE; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PROTEIN DETERMINATION; PULOMARY SURFACTANT; SHEEP; SURFACE TENSION; SURFACTANT;

OVIS; OVIS ARIES;

EID: 0034757481     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1510     Document Type: Conference Paper

References (37)

1. Pulmonary surfactant: Functions and molecular composition
2. Pulmonary surfactant in health and human lung diseases: State of the art
3. Surfactant and the adult respiratory distress syndrome
4. Quantitative analysis of pulmonary surfactant phospholipids by high-performance liquid chromatography and light-scattering detection
5. Surfactant: Composition, structure, and metabolism
6. Method of purification affects some interfacial properties of pulmonary surfactant proteins B and C and their mixtures with dipalmitoylphosphatidylcholine
7. Synthesis, processing and secretion of surfactant proteins B and C
8. Structure and properties of surfactant protein B
9. Hydrophobic surfactant-associated polypeptides: SP-C is a lipopeptide with two palmitoylated cysteine residues, whereas SP-B lacks covalently linked fatty acyl groups
10. Biophysical activity of an artificial surfactant containing an analogue of surfactant protein (SP)-C and native SP-B
11. Cell-specific posttranslational processing of the surfactant-associated protein SP-B
12. Two hydrophobic low-molecular-mass protein fractions of pulmonary surfactant
13. Quantitative analysis of pulmonary surfactant proteins B and C
14. An HPLC procedure for the analysis of proteins in lung lavage fluid
15. Structure-function relationships of bovine pulmonary surfactant proteins: SP-B and SP-C
16. Purification of a hydrophobic surfactant peptide using high-performance liquid chromatography
17. Purification of hydrophobic integral membrane proteins from Mycoplasma hyopneumoniae by reversed-phase high-performance liquid chromatography
18. Quantitative analysis of hydrophobic pulmonary surfactant proteins by high-performance liquid chromatography with light-scattering detection
19. Effects of a surfactant-associated protein and calcium ions on the structure and surface activity of lung surfactant lipids
20. Proteolytic inactivation of dog lung surfactant-associated proteins by neutrophil elastase
21. A simple method for the isolation and purification of total lipids from animal tissues
22. Investigation of over-compressed spread dipalmitoyl phosphatidylcholine films and the influence of solvent vapour in the gas phase on π-A isotherms measured by using the captive bubble technique
23. The influence of spreading solvent traces in the atmosphere on surface tension measurements by using a microfilm balance and the captive bubble method
24. A study of captive bubbles with axisymmetric drop shape analysis
25. Calcium ions and interactions of pulmonary surfactant proteins SP-B and SP-C with phospholipids in spread monolayers at the air/water interface
26. Cholesterol modifies the properties of surface films of dipalmitoylcholine plus pulmonary surfactant-associated protein B or C spread or adsorbed at the air-water interface
27. Interfacial behaviour and mechanical properties of spread lung surfactant protein/lipid layers
28. cDNA cloning of ovine pulmonary SP-A, SP-B, and SP-C: Isolation of two different sequences for SP-B
29. Ovine surfactant protein cDNAs: Use in studies on fetal lung growth and maturation after prolonged hypoxemia
30. Lung surfactant protein SP-C from human, bovine, and canine sources contains palmityl cysteine thioester linkagess
31. The proteins of the surfactant system
32. Combinations of fluorescently labeled pulmonary surfactant proteins SP-B and SP-C in phospholipid films
33. SP-B refining of pulmonary surfactant phospholipid films
34. Identification of a novel alternatively spliced mRNA of murine pulmonary surfactant protein B
35. An alternatively spliced surfactant protein B mRNA in normal human lung: Disease implication
36. Allelic heterogeneity in hereditary surfactant protein B (SP-B) deficiency
37. Fluorescently labeled pulmonary surfactant protein C in spread phospholipid monolayers