Sequence analysis of the S gene of recombinant MHV-2/A59 coronaviruses reveals three candidate mutations associated with demyelination and hepatitis

Journal of NeuroVirology

Volumn 7, Issue 5, 2001, Pages 432-436

  Das Sarma, J ^a   Fu, L ^a   Hingley, S T ^a   Lai, M M C ^a   Lavi, E ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

Coronavirus; Demyelination; Hepatitis; Mouse hepatitis virus (MHN); Multiple sclerosis (MS); Nidoviruses

Indexed keywords

VIRUS GLYCOPROTEIN;

ACUTE DISEASE; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BRAIN INFECTION; CHRONIC DISEASE; CONTROLLED STUDY; DEMYELINATION; ENCEPHALITIS; GENE SEQUENCE; HEPATITIS; LIVER; MENINGITIS; MOUSE; MURINE HEPATITIS CORONAVIRUS; NEUROTROPISM; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; SEQUENCE ANALYSIS; VIRUS GENE; VIRUS GENOME; VIRUS RECOMBINANT; VIRUS RECOMBINATION; VIRUS STRAIN;

AMINO ACID SUBSTITUTION; ANIMALS; BRAIN; CARDIOVIRUS INFECTIONS; DEMYELINATING DISEASES; ENCEPHALITIS, VIRAL; GENES, VIRAL; HEPATITIS, VIRAL, ANIMAL; LIVER; MALE; MEMBRANE GLYCOPROTEINS; MENINGITIS, VIRAL; MICE; MICE, INBRED C57BL; MURINE HEPATITIS VIRUS; POINT MUTATION; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS, RNA; SPECIES SPECIFICITY; SPINAL CORD; VIRAL ENVELOPE PROTEINS; VIRAL STRUCTURAL PROTEINS; VIRULENCE;

ANIMALIA; CORONAVIRUS; MURINAE; MURINE HEPATITIS VIRUS;

EID: 0034755810     PISSN: 13550284     EISSN: None     Source Type: Journal    
DOI: 10.1080/135502801753170282     Document Type: Article

References (36)

1. Murine hepatitis virus-4 (strain JHM)-induced neurologic disease is modulated in vivo by monoclonal antibody
2. Nidovirales: A new order comprising Coronaviridae and Arteriviridae
3. Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell-cell fusion
4. Site specific alteration of murine hepatitis virus type 4 peplomer glycoprotein S results in reduced neurovirulence
5. Mouse hepatitis virus type-2 infection in mice: An experimental model system of acute meningitis and hepatitis
6. Demyelination determinants map to the spike glycoprotein gene of coronavirus mouse hepatitis virus
7. Monoclonal antibodies to the matrix (E1) glycoprotein of mouse hepatitis virus protect mice from encephalitis
8. Alteration of the pH dependence of coronavirus-induced cell fusion: Effect of mutations in the spike glycoprotein
9. MHV-A59 fusion mutants are attenuated and display altered hepatotropism
10. Replication and plaque formation of mouse hepatitis virus (MHV-2) in mouse cell line DBT culture
11. Comparison of mouse hepatitis virus strains for pathogenicity in weanling mice infected by various routes
12. Pathogenesis of mouse hepatitis virus-induced demyelination
13. RNA recombination of murine coronavirus: Recombination between fusion-positive mouse hepatitis virus A59 and fusion-negative mouse hepatitis virus 2
14. Mouse hepatitis virus type 4 (JHM strain)-induced fatal central nervous system disease, part 1 (genetic control and the murine neurone as the susceptible site for disease)
15. Selected mutants of mouse hepatitis virus type 4 (JHM strain) induce different CNS diseases. Pathobiology of disease induced by wild type and mutants ts8 and ts15 in BALB/c and SJL/J mice
16. The molecular biology of coronaviruses
17. Single amino acid changes in the viral glycoprotein M affect induction of interferon by the coronavirus transmissible gastroenteritis virus
18. Limbic encephalitis following inhalation of murine coronavirus MHV-A59
19. The organ tropism of mouse hepatitis virus A59 is dependent on dose and route of inoculation
20. Experimental demyelination produced by the A59 strain of mouse hepatitis virus
21. Targeted recombination between MHV-2 and MHV-A59 to study neurotropic determinants of MHV
22. The pathogenesis of MHV nucleocapsid gene chimeric viruses
23. Determinants of coronavirus MHV pathogenesis are localized to 3′ portions of the genome as determined by ribonucleic acid-ribonucleic acid recombination
24. Nidovirus infections: Experimental model systems of human neurologic diseases
25. Coronaviruses
26. Altered pathogenesis phenotypes of murine coronavirus MHV-A59 are associated with a Q159L amino acid substitution in the receptor binding domain of the spike protein
27. Targeted recombination within the spike gene of murine coronavirus mouse hepatitis virus-A59: Q159 is a determinant of hepatotropism
28. The spike protein of murine coronavirus determines the ability of the virus to replicate in the liver and cause hepatitis
29. Identification of the spinal cord as a major site of persistence during chronic infection with a murine coronavirus
30. Pathogenesis of chimeric MHV-4/MHV-A59 recombinant viruses: The murine coronavirus spike protein is a major determinant of neurovirulence
31. A simple method of estimating fifty percent end points
32. The biology and pathogenesis of coronaviruses
33. Genetic variation of neurotropic and non-neurotropic murine coronaviruses
34. Pathogenesis of demyelination induced by a mouse hepatitis virus (JHM virus)
35. Acquired fusion activity of a murine coronavirus MHV-2 variant with mutations in the proteolytic cleavage site and the siganal sequence of the S protein
36. Heteroginity of gene expression of the hemagglutinin-esterase (HE) protein of murine coronaviruses